FastQCFastQC Report
Mon 9 Oct 2017
000000000-B4Y2B_l01n02_3006_d8_nw.3520000009f99f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y2B_l01n02_3006_d8_nw.3520000009f99f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105218
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG4830.4590469311334562No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA3060.2908247638236804No Hit
GAGCAAAAGCAGGGGAAGTGGGAGAGGGAAAAGGAGAAGGAAGCTTCCCT2830.2689653861506586No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG2520.23950274667832502No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA2410.22904826170427112No Hit
GAGCAAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCTATTCA2410.22904826170427112No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC1670.15871809006063603No Hit
GCCGTTACACCTTTGTTCGAGTCATGATTGGGCCATGAACTTGTCTTGGG1570.14921401281149613No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT1560.14826360508658215No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG1430.1359083046627003No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT1370.13020585831321638No Hit
GGGATGTACAGTGGGGGCCACTACTATGGAAAACCGCAGCTCCTCAAAAA1310.12450341196373245No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA1290.12260259651390445No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT1260.11975137333916251No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC1260.11975137333916251No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG1090.1035944420156247No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTGCG100.0070819096145.02
TATAATG203.8563422E-4108.752
GAGCAAA4350.0105.000011
GAGCGAA750.096.6666641
AGCAAAA5000.091.352
GCAAAAG6150.076.6260153
ATATAAT406.6355315E-572.51
CAAAAGC6850.068.795624
TCTATGT451.18891774E-464.444448
AAAAGCA7450.063.255035
CTCTAGC606.7634937E-660.416661
AGCGAAA1102.5465852E-1159.318182
TAATCAC502.0019492E-458.06
TAGCTCT502.0019492E-458.04
AAAGCAG9350.057.3796776
GCAGGTA1500.053.1666649
AGCTCTA553.205758E-452.7272725
AAGCAGG10200.052.5980387
CGAAAGC1351.8735591E-1048.3333324
GCGAAAG1351.8735591E-1048.3333322
Produced by FastQC (version 0.11.2)