FastQCFastQC Report
Mon 9 Oct 2017
000000000-B4Y2B_l01n02_3006_d5_nw.3520000009fa5b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y2B_l01n02_3006_d5_nw.3520000009fa5b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191712
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG18210.9498622934401603No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA16720.8721415456518111No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT13360.6968786513102987No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA11840.6175930562510432No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG7790.40633867467868473No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCT7620.3974712068102153No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG7260.37869303955933903No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG6740.3515690201969621No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC5360.2795860457352696No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT5270.2748915039225505No Hit
GGATAGCAGTTCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGG4960.2587214154565181No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT4920.256634952428643No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG4420.23055416458020364No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG4130.21542730762810883No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC3740.19508429310632616No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC3630.18934651977966951No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC3610.18830328826573195No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC3440.17943582039726258No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG3440.17943582039726258No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT3070.16013603738941745No Hit
GCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAAC2880.15022533800701052No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT2870.14970372225004172No Hit
GTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTTATC2820.1470956434651978No Hit
GGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTT2780.14500918043732267No Hit
TCCACCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCAT2770.14448756468035387No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT2640.13770655983975966No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA2620.13666332832582206No Hit
GAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA2570.13405524954097814No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA2410.12570939742947754No Hit
GCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGA2370.1236229344016024No Hit
GATCTTATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAA2300.1199716241028209No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA2280.11892839258888332No Hit
CTGCTATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCAGTATAAG2260.11788516107494576No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT2250.11736354531797696No Hit
GAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATAACCAT2240.11684192956100817No Hit
CTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCC2230.1163203138040394No Hit
GAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA2210.11527708229010182No Hit
AGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG2200.11475546653313302No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATG2140.11162577199132032No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG2110.11006092472041396No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC2100.10953930896344516No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT1990.10380153563678851No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG1940.10119345685194459No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC1940.10119345685194459No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG1930.10067184109497579No Hit
CACTAGACCAAAAGCAGCTTCTGTGGTCACTGTTCCCATCCTGTTGTATA1920.10015022533800699No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAACAC100.0070864237145.03
GAGCGAA1150.0132.391311
GATCTTA404.794856E-9108.751
GAGCAAA13150.0103.650191
ATCTTAT502.258821E-887.02
GCAAAAG16450.083.73863
GCAGGTA5100.082.450989
AGCAAAA16750.082.23882
AAGCAGG19100.080.8507847
AGCGAAA2600.075.288471
GCAGGGT5500.075.136379
CAAAAGC18450.074.661254
AAAGCAG20800.074.242796
AGTAGAA1300.072.51
AGCAGGG7900.072.58
GCAGGGG1800.072.499999
AGCAGGT8850.072.090398
GCGAAAG2750.071.1818162
AAAAGCA19950.070.137855
CGAAAGC2850.068.684213
Produced by FastQC (version 0.11.2)