FastQCFastQC Report
Mon 9 Oct 2017
000000000-B4Y2B_l01n02_3004_d8_nw.3520000009fbc3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y2B_l01n02_3004_d8_nw.3520000009fbc3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97223
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG7020.7220513664462113No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA4220.43405367042777937No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG3290.3383972928216574No Hit
GATACTGGGAAGGGAGACACGTCAGATCCCCAGAGAGGTTTAGGGGGGAC2880.29622620161895846No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA2550.2622836160167862No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC2490.2561122368163912No Hit
GAGCAAAAGCAGGGGAAGTGGGAGAGGGAAAAGGAGAAGGAAGCTTCCCT1970.20262695041296813No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC1930.1985126976127048No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG1750.1799985600115199No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT1690.17382718081112494No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC1610.16559867521059832No Hit
CCCCAACTGCATTTTTGACATCCTCATAAGTATGTCCTGGAAGAGAAGGT1580.16251298561040084No Hit
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT1570.161484422410335No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT1530.1573701696100717No Hit
CCCAATATCACCCCCACCATGCAGGTCCTTCTCCAAGGAGTTCACCACGG1480.15222735360974254No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT1370.14091315840901844No Hit
GCCGTTACACCTTTGTTCGAGTCATGATTGGGCCATGAACTTGTCTTGGG1340.13782746880882096No Hit
GGCAAGTGCACCAGTTGAATAGCTTAGTGACACCTCCTTGGCCCCATGGA1320.1357703424086893No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG1310.13474177920862349No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC1300.13371321600855765No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC1130.11622764160743858No Hit
GAGCAAAAGCAGGCAAGTGGGAGTTCAGAGAGGAGAACAGTTGCTGAGAG1120.11519907840737274No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT1000.10285632000658279No Hit
GCACTGCAGGGGGGGGTGCTCTGGGGGAATGAAAGAGGGAGGCCCCCGCA1000.10285632000658279No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA980.10079919360645115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA4300.0111.279071
AGCAAAA5350.088.084112
GAGCGAA1000.087.01
GCAAAAG5800.082.53
CAAAAGC6000.078.5416644
CCTTATA406.632993E-572.52
TAGACCT406.632993E-572.54
CCAATAT502.2905897E-672.52
CGTTACA607.955532E-872.53
GTTACAC607.955532E-872.54
GCAGGTA1300.072.59
AGCGAAA1550.065.483871
AAAGCAG8550.065.29246
AAAAGCA7250.065.000015
CCTACTA350.003556826262.1428575
GCGAAAG1650.061.5151522
TCTACGC606.75963E-660.416668145
TACAATC606.75963E-660.4166687
CGAAAGC1700.059.7058833
GCAGGGT2600.058.5576939
Produced by FastQC (version 0.11.2)