FastQCFastQC Report
Mon 9 Oct 2017
000000000-B4Y2B_l01n02_3003_d8_nw.3520000009f982.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y2B_l01n02_3003_d8_nw.3520000009f982.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138774
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG12910.9302895355037687No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA8790.6334039517488866No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG6270.4518137403259977No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA3380.24356147405133524No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC3350.24139968582011037No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT3260.23491432112643576No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC2750.198163921195613No Hit
GAGCAAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCTATTCA2530.18231080749996398No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG2510.18086961534581406No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC2490.17942842319166413No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT2450.17654603888336431No Hit
GGCAAGTGCACCAGTTGAATAGCTTAGTGACACCTCCTTGGCCCCATGGA2430.1751048467292144No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG2330.16789888595846483No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT2120.15276636833989077No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG2020.1455604075691412No Hit
GGTCCAATCGCACGCAAAGAGGGCCTATTATCTTTTGCCTAGGCATGAGC1990.14339861933791634No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC1940.13979563895254155No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT1760.12682490956519232No Hit
GCCGTTACACCTTTGTTCGAGTCATGATTGGGCCATGAACTTGTCTTGGG1740.1253837174110424No Hit
CCCCAACTGCATTTTTGACATCCTCATAAGTATGTCCTGGAAGAGAAGGT1640.11817775664029284No Hit
GGGATGTACAGTGGGGGCCACTACTATGGAAAACCGCAGCTCCTCAAAAA1620.11673656448614293No Hit
GCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAAC1610.11601596840906797No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC1520.10953060371539337No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA1490.1073688154841685No Hit
GAGCAAAAGCAGGGGAAGTGGGAGAGGGAAAAGGAGAAGGAAGCTTCCCT1460.10520702725294363No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT1460.10520702725294363No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG100.0070843287145.01
CGCCTAA100.0070843287145.09
GAGCAAA6350.0113.0314941
GAGCGAA1300.094.807691
AGCAAAA7850.090.509552
CAGCGCC353.426832E-582.857149
GCAGGTA2950.078.6440669
GCAAAAG9750.073.615393
GCAGCGC406.642997E-572.58
GCAGGGT5050.071.782189
AGCGAAA1850.066.621622
CAAAAGC11350.063.237894
AAAGCAG14050.060.8896836
AAAAGCA12150.059.670785
CGAAAGC2100.058.6904754
AAGCAGG15400.056.0227287
GCGAAAG2200.056.0227243
CAAGGTA400.006039951554.3754
GTATTAA553.2093332E-452.7272721
TATGTTG1101.6570993E-952.7272726
Produced by FastQC (version 0.11.2)