FastQCFastQC Report
Mon 9 Oct 2017
000000000-B4Y2B_l01n02_3003_d-2_nw.3520000009f8e0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y2B_l01n02_3003_d-2_nw.3520000009f8e0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124211
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT3790.3051259550281376No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA2790.2246177874745393No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA2520.20288058223506775No Hit
AGCAAAAGCAGGCAGGTGTTGGCTGGCAGTGGGACCAGTGGACTGAGTCA2350.18919419375095603No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG2100.16906715186255644No Hit
GGGGAAGTGGGAGAGGGAAAAGGAGAAGGAAGCTTCCCTCGGAGCAGGAA1820.14652486494754893No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG1600.1288130680857573No Hit
TTCTTATACATGCCCTTAAGGACCATCTGAATGTGAAGACTCGAGAATTA1400.11271143457503763No Hit
GGTGCACTTGCCAGTTGCATGGGCCTCATATACAACAGGATGGGAACAGT1350.10868602619735772No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA1300.10466061781967781No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGGTG100.007083441144.999985
AAACCGC259.3672634E-487.05
AACCGCT300.001931739372.56
ACCGTCT406.640256E-572.499998
GAGCGAA1250.069.61
TACTATA651.0880465E-555.7692342
AGCGAAA1600.054.3749962
GAGCAAA2600.052.980771
GCGAAAG1650.052.7272723
CGAAAGC1700.051.1764684
GCCTCGA901.3141507E-648.3333327
TGTGACA803.715675E-545.3124969
CTTCGTC657.311697E-444.6153879
CCGTCTG657.311697E-444.6153879
TATAGAG657.311697E-444.6153872
GGCCTCG1002.7147526E-643.56
GCAAAAG3300.041.7424243
AGCAAAA3350.041.1194042
GGAGCGA907.444598E-540.277781
CCTCGAT907.444598E-540.277788
Produced by FastQC (version 0.11.2)