FastQCFastQC Report
Mon 9 Oct 2017
000000000-B4Y2B_l01n02_3002_d8_nw.3520000009fd07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y2B_l01n02_3002_d8_nw.3520000009fd07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32518
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA990.304446767943908No Hit
GAGCAAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCTATTCA930.2859954486745802No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG760.23371671074481826No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG690.2121901715972692No Hit
GGGATGTACAGTGGGGGCCACTACTATGGAAAACCGCAGCTCCTCAAAAA680.20911495171904793No Hit
GAGCAAAAGCAGGGGAAGTGGGAGAGGGAAAAGGAGAAGGAAGCTTCCCT590.18143797281505628No Hit
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT590.18143797281505628No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT560.1722123131803924No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG430.1322344547635156No Hit
GGTCTGGCCCCTGGGTCCTGCTCCCTCTCCTGCCCTCTAGGCACCTGCCT410.126084015007073No Hit
GCACTGCAGGGGGGGGTGCTCTGGGGGAATGAAAGAGGGAGGCCCCCGCA360.11070791561596655No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG330.10148225598130266No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACA100.007059553145.03
TGTAGAG100.007059553145.02
TTTCGCT203.832122E-4108.753
TTCGCTC203.832122E-4108.754
GCTTTCG203.832122E-4108.751
GAGCGAA405.9845297E-790.6251
TCGCTCC259.304656E-487.05
GAGCAAA1000.087.01
CTTTCGC259.304656E-487.02
TATAATG259.304656E-487.02
TATATGT451.2061555E-680.555564
AGCGAAA451.2061555E-680.555562
CGCTCCC300.001918875572.56
CGAAAGC553.973959E-665.909094
GTATGTA553.973959E-665.909099
ATGTATG651.0701562E-555.769237
GCAAAAG1600.054.3753
AGCAAAA1650.052.7272762
GCGAAAG701.6596465E-551.785713
ATATATG858.6583896E-751.1764683
Produced by FastQC (version 0.11.2)