FastQCFastQC Report
Mon 9 Oct 2017
000000000-B4Y2B_l01n02_3002_d-2_nw.3520000009fe57.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y2B_l01n02_3002_d-2_nw.3520000009fe57.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164058
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT6050.3687720196515866No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA5040.3072084262882639No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG4550.27734094039912716No Hit
GGTCTATACTACCCAAAATCTACATATTTGATGTAATACCTATCAAAATA2170.1322702946518914No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACCTCTTAACAATA1880.11459361932974924No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2450.079.897961
CGAAAGC2550.079.607843
GCGAAAG2600.078.076932
GAGCAAA2500.058.01
ATGTCGT400.00604190954.3756
TGTCGTG450.00962488848.3333327
ATATAAG657.3185936E-444.6153873
GCAAAAG3700.043.108113
CAAAAGC4200.041.428574
CGTACAC907.455079E-540.277783
ATCCCTA750.001479770438.6666649
AGACCTC1901.2732926E-1038.1578945
AGCAAAA4300.037.093022
CATACTC1001.3863854E-436.255
GAAAGCA5950.035.3361324
AACTGGT850.002735340334.117657
TATGAGC1301.6463984E-533.461546
TATAGAC1956.9157977E-933.461542
ACATGCC1601.8644951E-631.718758
GTACACC1204.0434208E-430.2083344
Produced by FastQC (version 0.11.2)