FastQCFastQC Report
Mon 9 Oct 2017
000000000-B4Y2B_l01n02_3001_d8_nw.3520000009fa17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y2B_l01n02_3001_d8_nw.3520000009fa17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98207
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG7430.7565652142922602No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA4400.44803323592004646No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA3780.3849012799494944No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG2870.2922398607023939No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG2590.2637286547802091No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT2280.23216267679493316No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC2090.2128157870620221No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT1990.20263321351838465No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT1980.2016149561640209No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG1960.1995784414552934No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC1880.19143238262038348No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC1680.17106723553310862No Hit
GATACTGGGAAGGGAGACACGTCAGATCCCCAGAGAGGTTTAGGGGGGAC1560.15884814728074373No Hit
AGATCACACTGAAGTTCGCTTTCAGTACTATGTTCTTTTCCATGATCGCC1520.15477511786328876No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG1440.14662905902837883No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCT1370.13950125754783263No Hit
GCCGTTACACCTTTGTTCGAGTCATGATTGGGCCATGAACTTGTCTTGGG1220.1242273972323765No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC1190.12117262516928527No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT1180.12015436781492154No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT1120.11404482368873908No Hit
GTGTAATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACT1030.10488050749946543No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA990.10080747808201045No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA990.10080747808201045No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA1000.0108.751
GAGCAAA4000.0105.1251
AGTAGGG259.361379E-487.08
AACAGGT259.361379E-487.07
AGCAAAA5400.076.527782
AAGGTCG300.001930530272.59
GATAGCA607.9564416E-872.56
GCAGGGT3050.068.9344259
AGCGAAA1700.068.235291
GGATAGC651.3849785E-766.923075
GCGAAAG1850.066.621633
GCAAAAG6450.066.317833
CAAAAGC6850.065.620444
AAAGCAG9050.064.0883946
CGAAAGC2050.063.6585354
ATCTAGG350.00355693662.142857145
CTATTAA350.00355693662.1428571
AAGCAGG9650.061.606227
AAAAGCA7750.058.9354825
GTGTAAT902.0241714E-856.3888851
Produced by FastQC (version 0.11.2)