FastQCFastQC Report
Mon 9 Oct 2017
000000000-B4Y2B_l01n02_3001_d5_nw.3520000009fac4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y2B_l01n02_3001_d5_nw.3520000009fac4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34739
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG1620.4663346670888626No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC1570.4519416218083422No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA1450.4173983131350931No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA1160.3339186505080745No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG1130.3252828233397622No Hit
TCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTTCGTC980.28210368749820086No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT960.27634646938599267No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA910.26195342410547223No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG880.25331759693716No Hit
GGGTTTCGACTTTCTCTTACTTGATCCATCATTGCCCTCTGGGCAGCTGT840.2418031607127436No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA730.21013846109559858No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG650.18710958864676588No Hit
CTGTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACT590.16983793431014135No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG570.16408071619793316No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC540.1554448890296209No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC520.1496876709174127No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC510.1468090618613086No Hit
GAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA510.1468090618613086No Hit
CCCTTCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTT510.1468090618613086No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCATGTCAAAG500.14393045280520453No Hit
GGAATCATGTCCATTATTCTCTTGTCTGCTGTAATTGGGTATCTCATTGC500.14393045280520453No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT500.14393045280520453No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA480.13817323469299633No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT460.13241601658078817No Hit
CTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCC430.1237801894124759No Hit
TCTCTTACTTGATCCATCATTGCCCTCTGGGCAGCTGTTTGAAATTTTCC430.1237801894124759No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT420.1209015803563718No Hit
ACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAATA410.11802297130026772No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT400.11514436224416362No Hit
GCTTTACCCCATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTAT400.11514436224416362No Hit
GTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTTATC400.11514436224416362No Hit
GCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGA380.10938714413195544No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT370.10650853507585135No Hit
GAGTAAGACCTGCTGTTGCATCTTCGCCATTGTTTGCTTGGCGCCAAACT370.10650853507585135No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA370.10650853507585135No Hit
GTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTCTGCATTGTCT360.10362992601974726No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG360.10362992601974726No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG360.10362992601974726No Hit
GGGTTTGTCCTTGTTCATTCCTCTCTGGAATCATGTCCATTATTCTCTTG350.10075131696364317No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG350.10075131696364317No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTATA151.2198773E-4145.04
CCTACTA151.2198773E-4145.05
CACCTAC100.007061603145.03
CTAGTGC100.007061603145.09
GGTGCCA100.007061603145.08
TTTATAG151.2198773E-4145.05
GAGCGAA301.0792246E-7120.8333361
TTATGGG203.8343406E-4108.753
TTATAGC203.8343406E-4108.756
GCAGGGT554.0017767E-1092.272729
GAGCAAA1050.089.761911
GTGTTTA259.310023E-487.02
TATAGCA259.310023E-487.07
AGCGAAA451.2078945E-680.555562
GCAGGTA451.2078945E-680.555569
TGTTTAT300.001919978472.53
GCGAAAG502.2595996E-672.53
CTCCCAA300.001919978472.51
AGCAGGT702.7066562E-972.499998
AAGCAGG1950.070.641037
Produced by FastQC (version 0.11.2)