FastQCFastQC Report
Mon 9 Oct 2017
000000000-B4Y2B_l01n02_3001_d-2_nw.3520000009f918.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y2B_l01n02_3001_d-2_nw.3520000009f918.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140388
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA3980.2835000142462319No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG3270.23292589110180356No Hit
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT2010.14317463031028294No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG2350.098.7234042
AGCGAAA2400.096.6666641
CGAAAGC2600.089.2307743
GAGCAAA1900.061.0526351
GTATATG606.7752553E-660.4166681
GAAAGCA4200.055.2380944
GGAGCGA400.006040096754.3751
GAGCGAA805.83721E-754.3751
TACATAC1900.045.7894746
CATACAT2100.044.880958
TAATAGC657.314962E-444.6153876
AAAGCAG8050.042.329195
TACATGG750.001479040638.6666686
GCAAAAG3500.037.2857133
GTATATA2600.036.251
GGTCTAT1209.505398E-636.251
TATAATA1001.3853644E-436.252
AGCAAAA3450.035.724642
TATACAT3050.035.6557354
ACATACA2650.035.566047
Produced by FastQC (version 0.11.2)