FastQCFastQC Report
Mon 9 Oct 2017
000000000-B4Y2B_l01n01_ny238_plasmid_mix.3510000009feb6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y2B_l01n01_ny238_plasmid_mix.3510000009feb6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149041
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCCAG4310.28918217134882346No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT3800.2549633993330694No Hit
CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT3180.21336410786293702No Hit
GTATATGAAACAGCCCCCTGCTGTCCATTCCTTATTCCATAGAAAAGCCT3180.21336410786293702No Hit
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAA2690.18048724847525177No Hit
ATTAATAACTAGTCAATAATCAATGTCGACCCAGGTGGCACTTTTCGGGG2570.17243577270683907No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG2470.16572620956649514No Hit
AACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAG2100.14090082594722259No Hit
TCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATAC1940.13016552492267228No Hit
GTCCTGGGGTTGACCAGAGGGCCCCGGGCGCTCCGTGTGTGGCTGCGATG1940.13016552492267228No Hit
GTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACT1900.1274816996665347No Hit
CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC1860.12479787441039714No Hit
TGGTAATAGCGATGACTAATACGTAGATGTACTGCCAAGTAGGAAAGTCC1750.11741735495601881No Hit
CCCATATGTCCTTCCGAGTGAGAGACACAAAAAATTCCAACACACTATTG1740.11674639864198441No Hit
CCCCAGGACACACGCGGGAGCAGCGCCGGGCCGGGGACGCCCTCCCGGCC1720.11540448601391565No Hit
GAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTT1700.11406257338584685No Hit
CCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGG1680.11272066075777806No Hit
ATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAAT1680.11272066075777806No Hit
GTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATG1640.11003683550164049No Hit
GTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGCAT1630.1093658791876061No Hit
GTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT1630.1093658791876061No Hit
ATCTTATCATGTCTGGATCCGCTGCATTAATGAATCAGGGGATAACGCAG1610.1080239665595373No Hit
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA1610.1080239665595373No Hit
ATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTT1570.10534014130339975No Hit
TGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATC1550.10399822867533096No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGTAT406.644608E-572.51
AGATTAA350.003560551762.1428575
TACTAAA350.003560551762.1428579
TGGGCCC502.0046718E-458.06
CCATATT651.0891088E-555.7692344
CATATTA701.6889686E-551.7857135
TTACTGA450.0096231748.3333322
TCAGCCT450.0096231748.3333323
ATGTAAT803.7192756E-545.31253
ATACATC803.7192756E-545.31253
GAATATG657.3164224E-444.6153871
GACCTTA657.3164224E-444.6153877
TGTATAC1252.698198E-740.63
TTCTAAT907.451781E-540.2777752
ATCCCAT907.451781E-540.2777751
TACTGAT1105.234173E-639.5454567
TAAGATA1105.234173E-639.5454564
TCCTCCT1157.101913E-637.826084145
TGTATCT1209.50934E-636.252
TATCTTA1051.8461744E-434.5238084
Produced by FastQC (version 0.11.2)