FastQCFastQC Report
Mon 9 Oct 2017
000000000-B4Y2B_l01n01_extraction_neg_ctrl.3510000009fe9d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y2B_l01n01_extraction_neg_ctrl.3510000009fe9d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18319
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT850.4639991265898794No Hit
CCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCC700.38211692777990064No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGG510.2783994759539276No Hit
AGCGAAAGCAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACAC480.26202303619193185No Hit
ATACAGGCCTACCTCAAATAAACAAAGAAAAGTCTCAAATATACTATCTA460.25110540968393474No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAGCTCTATGCTGACAAAATGACC450.24564659642993614No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGT420.22927015666794037No Hit
CTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTT420.22927015666794037No Hit
CTTCTCTGTTGTCCAGTTTGTTGGCAGATGATTTTTTGTTCCATAATGCT420.22927015666794037No Hit
TCCTAATCCAATTGTATTTTCACTTTCACCCCTACAAGATCCTCCTAATT410.2238113434139418No Hit
GGCCTGTATTGATACAAACTTCCCTCTTCAAACTGCCTTTGCTATGTCCT380.20743490365194606No Hit
TTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGT370.20197609039794748No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT370.20197609039794748No Hit
AATCAGACATGAGAACAGAATGGTTTTAGCCAGCACTACAGCTAAGGCTA370.20197609039794748No Hit
ATTATAACCAAAGCATTATGGAACAAAAAATCATCTGCCAACAAACTGGA370.20197609039794748No Hit
TTTCTGGGCAGGCTGTTCTTGGGTGTGATCGAGAGGCTGGCTCAGGTGGC350.19105846388995032No Hit
TATTTAAAGCGACGGTAAATGCATTTGAAAAAAAGACGATCAAGAATCCA340.18559965063595174No Hit
GATAAGGGGCGTCTGTCAGGGCACCTCAGACCTCAAAGACGAGCTGAAGC340.18559965063595174No Hit
AAGCAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTT340.18559965063595174No Hit
GGATACAATGGGCAAAGAAAGGGGGTGTCAGAGGGGAATGCCAGGTTTCT340.18559965063595174No Hit
CTTTAGGACATAGCAAAGGCAGTTTGAAGAGGGAAGTTTGTATCAATACA330.18014083738195316No Hit
GGCTAGACAAATGGTGCAAGCGATGAGAACCATTGGGACTCATCCTAGCT330.18014083738195316No Hit
GCTAAAGGTTTATCAGTTTTATCTTTTCTAAGAACTGGCACTTGGGTTAA330.18014083738195316No Hit
AGATTACATTGCATCTGTAGATTGAAATCACCTGGAAAGCCACCTGGCCC330.18014083738195316No Hit
ATCCTAATCCAATTGTATTTTCACTTTCACCCCTACAAGATCCTCCTAAT320.17468202412795458No Hit
AAAGCAGGCAGGTGTTGGCTGGCAGTGGGACCAGTGGACTGAGTCAGGAG310.169223210873956No Hit
GCTTTGGGCTTTGTTTTTCTTTTTCCAATTCTTTCACATGTAAGGTTAGA290.15830558436595885No Hit
GTGTTAAATGATGGGGCTTTCTGGGCAGGCTGTTCTTGGGTGTGATCGAG290.15830558436595885No Hit
TGGCTGGATCGAGTGAGCAAGCAGCAGAGGCCATGGAGGTTGCTAGTCAG290.15830558436595885No Hit
ATCAGAAACGAATGGGGGTGCAGATGCAACGGTTCAAGTGATCCTCTCGC280.15284677111196027No Hit
ATTTAACACCTAGGCGCCCAGAAATGTTTTCAGAAAGATGCCTGCAACTC280.15284677111196027No Hit
ACCTTTAGCTATTCATCTAGAAAAAAGAAAGGACTCAAATAAATAAAATC280.15284677111196027No Hit
GAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAA280.15284677111196027No Hit
ATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGTTCCTT280.15284677111196027No Hit
GGTCTATGGTAGAAGGAAACGTATATAAGAAACTGAAATGAGGCCCATGT270.14738795785796166No Hit
ATGTAATCTCTATCAAAAAACCAAGAGCATCGGAGGCCTGGGTGGCTCAA270.14738795785796166No Hit
CTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTTTGGCACTCCTTCCG270.14738795785796166No Hit
GCAGAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAA260.1419291446039631No Hit
TTATTATTCTTCCTACTAGCTTTGGGCTTTGTTTTTCTTTTTCCAATTCT260.1419291446039631No Hit
TTTAACACCTAGGCGCCCAGAAATGTTTTCAGAAAGATGCCTGCAACTCA260.1419291446039631No Hit
CTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTG260.1419291446039631No Hit
GTAACAACCAACACCAAAGAAATCAAAGCATTATAACCAAAGCATTATGG260.1419291446039631No Hit
CTATTCAAGTGTGCGATAAGTATGTTTCACCTAAGAGGTCTGAGAAGGCT250.13647033134996453No Hit
GTTTTAGCCAGCACTACAGCTAAGGCTATGGAGCAAATGGCTGGATCGAG250.13647033134996453No Hit
ATTCTGTTCATCGTGATTCTGTTGGCTTTTCACGTCCTCAGTATTTCGTG250.13647033134996453No Hit
GGTTATAATGCTTTGATTTCTTTGGTGTTGGTTGTTACTTCTGCTTCATT250.13647033134996453No Hit
ATATCAGGCAGTCCCAGGACAAGAAGCCCGAGGAACCCACCAGCTCAGGT250.13647033134996453No Hit
GGTATGTGCAACCTGTGAACAGATTGCTGACTCCCAGCATCGGTCTCATA250.13647033134996453No Hit
CTGTTGTCCAGTTTGTTGGCAGATGATTTTTTGTTCCATAATGCTTTGGT240.13101151809596592No Hit
GATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACAT240.13101151809596592No Hit
GTCAAGTGCAAGATCCCAATGATATTTGCGGCAATAGCGAGAGGATCACT240.13101151809596592No Hit
GCCCTAGACTTTTGTTTGTTTGGAGGCTTTTGATTGCTGATTCCAGTTTC240.13101151809596592No Hit
CTTCTAACCGAGGTCGAAACGTACGTACTCTCTATCATCCCGTCAGGCCC240.13101151809596592No Hit
CAATTAACCCAAGTGCCAGTTCTTAGAAAAGATAAAACTGATAAACCTTT240.13101151809596592No Hit
TCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTT240.13101151809596592No Hit
CACTTGAACCGTTGCATCTGCACCCCCATTCGTTTCTGATAGGCCTGCAA240.13101151809596592No Hit
ATTGGATACAATGGGCAAAGAAAGGGGGTGTCAGAGGGGAATGCCAGGTT240.13101151809596592No Hit
ACCCAGGCCTCCGATGCTCTTGGTTTTTTGATAGAGATTACATTGCATCT240.13101151809596592No Hit
CCCAGATGCAGGGCAGATGCAACTCAAGCCCCAGTGCTATGGACCGAGAC230.12555270484196737No Hit
CTCCAGTGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGGCCTATC230.12555270484196737No Hit
GAGCTAGGATGAGTCCCAATGGTTCTCATCGCTTGCACCATTTGTCTAGC230.12555270484196737No Hit
CTGCAGAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAA230.12555270484196737No Hit
GTATTCTGCAGAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAAC230.12555270484196737No Hit
CCATAGACCTTTGTAAATTTTCCTCCCTTTCTCTGAAACATTTCCTGTTT230.12555270484196737No Hit
ACCATAGACCTTTGTAAATTTTCCTCCCTTTCTCTGAAACATTTCCTGTT230.12555270484196737No Hit
TTCTAACCGAGGTCGAAACGTACGTACTCTCTATCATCCCGTCAGGCCCC230.12555270484196737No Hit
GGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTCTGCAGAAGA220.12009389158796878No Hit
CCCCTACAAGATCCTCCTAATTGGCCCCCCTATTCCAAGCACATGGGCCT220.12009389158796878No Hit
CCTTTCAGTCCGTATTTAAAGCGACGGTAAATGCATTTGAAAAAAAGACG220.12009389158796878No Hit
TTTCTGATAGGCCTGCAAATTTTCAAGAAGATCATTTTTCAGACCAGCAC210.11463507833397019No Hit
GGTGTTAAATGATGGGGCTTTCTGGGCAGGCTGTTCTTGGGTGTGATCGA210.11463507833397019No Hit
ATTCAAGTGTGCGATAAGTATGTTTCACCTAAGAGGTCTGAGAAGGCTCA210.11463507833397019No Hit
ACCCAGAGTCCATCTGAAAGCCACCTGAGCCAGCCTCTCGATCACACCCA210.11463507833397019No Hit
GTATATTTGAGACTTTTCTTTGTTTATTTGAGGTAGGCCTGTATTGATAC210.11463507833397019No Hit
TCTGATAGGCCTGCAAATTTTCAAGAAGATCATTTTTCAGACCAGCACTG210.11463507833397019No Hit
AGCGAAAGCAGGGGGAGCAGCAGGCAGAGGGAGAAGAGGGAGAAGCAGGC210.11463507833397019No Hit
ATTCTGCAGAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCG210.11463507833397019No Hit
GTAGAAACAAGGTAGTTTTTTACTCCAGCTCTATGCTGACAAAATGACCA210.11463507833397019No Hit
AAACAAGGTAGTTTTTTACTCCAGCTCTATGCTGACAAAATGACCATCGT200.10917626507997162No Hit
CTCATAGACTTTGGCACTCCTTCCGTAGAAGGCCCTCCTTTCAGTCCGTA200.10917626507997162No Hit
TCCTAAAACATACAATCTTCCGAAACTGGAATCAGCAATCAAAAGCCTCC200.10917626507997162No Hit
GAAGAACACTGATCTTGAGGTTCTCATGGAATGGCTAAAGACAAGACCAA200.10917626507997162No Hit
CCCCTGAGATGGGGGTGTCCCCTTGAGGTTTAGGGGTGGGGGAGGGTCTC200.10917626507997162No Hit
AAAGCAGGGGGAGCAGCAGGCAGAGGGAGAAGAGGGAGAAGCAGGCTCCC200.10917626507997162No Hit
TGTCAGGGCACCTCAGACCTCAAAGACGAGCTGAAGCGGTATATCAGGCA200.10917626507997162No Hit
TGTCTAGCCTGACTAGCAACCTCCATGGCCTCTGCTGCTTGCTCACTCGA200.10917626507997162No Hit
GCCCAAAGCTAGTAGGAAGAATAATAAAGATGAGTAGAAATAAAACAGAA200.10917626507997162No Hit
GTCTGAGAAGGCTCAATGCGGTTATGCTTAACTGACATCTGAAGGATCTC200.10917626507997162No Hit
GAATAGGGGGGCCAATTAGGAGGATCTTGTAGGGGTGAAAGTGAAAATAC200.10917626507997162No Hit
TCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTA200.10917626507997162No Hit
GGCATATATATTATCACAATATGCAAAGAACCTTTACGGTGACCGCTACA200.10917626507997162No Hit
GGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTACT190.10371745182597303No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC190.10371745182597303No Hit
TCAGAGGGGAATGCCAGGTTTCTCTGACCCCAAAACTAGCCATTCCCCAC190.10371745182597303No Hit
GCATCTGGGAGGTCGCGGAGAAACATCTTGAGTTGCAGGCATCTTTCTGA190.10371745182597303No Hit
GAAGTAACAACCAACACCAAAGAAATCAAAGCATTATAACCAAAGCATTA190.10371745182597303No Hit
ATCCCAATGATATTTGCGGCAATAGCGAGAGGATCACTTGAACCGTTGCA190.10371745182597303No Hit
CCCAGAGTCCATCTGAAAGCCACCTGAGCCAGCCTCTCGATCACACCCAA190.10371745182597303No Hit
AGCGAAAGCAGGCAGGTGTTGGCTGGCAGTGGGACCAGTGGACTGAGTCA190.10371745182597303No Hit
GTGTTGGCTGGCAGTGGGACCAGTGGACTGAGTCAGGAGAAATTTAAGAA190.10371745182597303No Hit
AGCGAAAGCAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCC190.10371745182597303No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCAACC100.0070344843145.00002145
AACTGAC259.239129E-487.07
CTGACAT259.239129E-487.09
TGCTTAA259.239129E-487.02
CTTAACT259.239129E-487.04
GCTTAAC259.239129E-487.03
AGCTCTA554.1574822E-879.090915
CTCTATG554.1574822E-879.090917
TCTATGC554.1574822E-879.090918
CTATGCT554.1574822E-879.090919
GCTCTAT554.1574822E-879.090916
TATAATG406.490085E-572.500014
CAGCTCT607.599374E-872.54
CTCCAGC607.599374E-872.51
ATGCTTA300.001905411772.51
GAAAGCA300.001905411772.51
TTATAAT451.1629319E-464.444443
GGTTATA451.1629319E-464.444441
GTTATAA451.1629319E-464.444442
ACTGACA350.003510826762.1428578
Produced by FastQC (version 0.11.2)