000000000-B4Y2B_l01n01_3027_d1_nw.3510000009fb5d.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	000000000-B4Y2B_l01n01_3027_d1_nw.3510000009fb5d.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	82016
Sequences flagged as poor quality	0
Sequence length	151
%GC	44

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA	605	0.7376609442060086	No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG	587	0.7157140070230199	No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG	365	0.44503511509949273	No Hit
ATGATGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT	313	0.3816328521264144	No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT	257	0.31335349200156065	No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG	176	0.2145922746781116	No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT	171	0.20849590323839248	No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA	155	0.18898751463129146	No Hit
TCCACCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCAT	153	0.18654896605540383	No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT	150	0.18289114319157238	No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT	147	0.17923332032774095	No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATG	143	0.17435622317596566	No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT	140	0.1706984003121342	No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC	138	0.16825985173624658	No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA	138	0.16825985173624658	No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG	131	0.15972493172063987	No Hit
GTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTTATC	131	0.15972493172063987	No Hit
AACTCATCCTTTATGACAAAGAAGAAATAAGGAGAGTTTGGCGCCAAGCA	129	0.15728638314475224	No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC	124	0.15119001170503318	No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA	117	0.14265509168942644	No Hit
GAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATAACCAT	116	0.14143581740148264	No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC	115	0.14021654311353882	No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT	114	0.138997268825595	No Hit
ATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCCAAGGAGGTGT	113	0.1377779945376512	No Hit
ATATACAGTAAAGACAACAGTGTAAGAATCGGTTCCAAGGGGGATGTGTT	113	0.1377779945376512	No Hit
GCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAAC	112	0.13655872024970736	No Hit
CTTTCCGTCTACTCTTCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCT	111	0.13533944596176356	No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC	108	0.1316816230979321	No Hit
GTCCTACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCAG	106	0.12924307452204448	No Hit
TCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGAT	106	0.12924307452204448	No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT	104	0.12680452594615685	No Hit
GCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGGCG	100	0.12192742879438159	No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA	100	0.12192742879438159	No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA	99	0.12070815450643778	No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC	94	0.11461178306671868	No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC	89	0.10851541162699961	No Hit
ACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAATA	89	0.10851541162699961	No Hit
GTTAGTGGATGGGCTATATACAGTAAAGACAACAGTGTAAGAATCGGTTC	88	0.1072961373390558	No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG	88	0.1072961373390558	No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG	87	0.10607686305111198	No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG	87	0.10607686305111198	No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC	86	0.10485758876316817	No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAGTGAAC	85	0.10363831447522436	No Hit
AGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAA	84	0.10241904018728054	No Hit
TTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCCC	83	0.10119976589933671	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTAGAT	10	0.0070790797	145.00002	2
AGTAGAA	15	1.2259235E-4	145.0	1
GAGCGAA	50	0.0	130.5	1
TTATACT	20	3.8532735E-4	108.75001	4
TCCACCT	40	6.067221E-7	90.62501	1
GCAGGTA	120	0.0	90.625	9
GTGTTGG	25	9.3558274E-4	87.0	1
CCACCTA	45	1.2227829E-6	80.55556	2
CACCTAC	45	1.2227829E-6	80.55556	3
GCAGGGT	250	0.0	75.4	9
GAGCAAA	415	0.0	75.12048	1
CTACACT	40	6.626803E-5	72.50001	4
ATTAGAA	40	6.626803E-5	72.50001	3
TCTACAC	40	6.626803E-5	72.50001	3
CTACGTC	30	0.0019293894	72.5	9
CCTTATA	30	0.0019293894	72.5	2
ACTACGT	30	0.0019293894	72.5	8
AGCGAAA	95	5.456968E-12	68.68421	1
CCTACTA	55	4.028476E-6	65.909096	5
GCGAAAG	100	9.094947E-12	65.25	2

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content