FastQCFastQC Report
Mon 9 Oct 2017
000000000-B4Y2B_l01n01_3025_d-2_nw.3510000009fe4d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y2B_l01n01_3025_d-2_nw.3510000009fe4d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166845
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT1760.10548712877221374No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2150.0118.0232541
GCGAAAG2450.0103.571432
CGAAAGC2900.090.03
ATGGCCG300.001932905972.58
GTATAAG400.00604208654.3751
CGCAGAT450.009625168548.333336145
ACTCCGA450.009625168548.3333368
GAAAGCA5650.046.194694
TATAGAC1151.403132E-744.1304362
GAGCAAA4150.043.6746981
GCAAAAG4300.042.151163
TGCTAGG700.001054108841.428572
CCTACAC750.00147984238.6666643
TAGGTAC1157.107017E-637.826088145
CAAAAGC4650.037.4193534
AGCAAAA5100.036.9607852
ATAGACA1209.5161595E-636.253
GTCTAGG850.002735471134.117651
TACTAAT850.002735471134.117652
AAAGCAG9650.033.808295
Produced by FastQC (version 0.11.2)