FastQCFastQC Report
Mon 9 Oct 2017
000000000-B4Y2B_l01n01_3024_d-2_nw.3510000009fc4f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y2B_l01n01_3024_d-2_nw.3510000009fc4f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112961
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA2700.23902054691442182No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG2140.189445914961801No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGAC256.439881E-6116.03
AGCGAAA1650.079.090911
GAGCAAA3750.073.466671
GCGAAAG1700.072.52
CACCGCA300.001931284272.59
CGAAAGC1850.070.540543
AGCAAAA4550.060.5494542
CAAAAGC4700.057.074474
GTCATAC400.00603705454.3751
GCAAAAG5150.052.087383
GAGCGAA1004.6535206E-850.751
GGAGCGA450.00961718448.3333321
AAAAGCA6400.043.0468755
GTATCTG700.001052661641.428579
CAGCTCG951.0231106E-438.157899
GAAAGCA3400.036.254
TTAGTGG800.002028989636.252
AAAGCAG9050.035.248626
AAGCAGG9850.033.121837
AGCAGGT2008.80209E-932.6258
Produced by FastQC (version 0.11.2)