FastQCFastQC Report
Mon 9 Oct 2017
000000000-B4Y2B_l01n01_3022_d-2_nw.3510000009f95f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y2B_l01n01_3022_d-2_nw.3510000009f95f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160380
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA2850.17770295548073325No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT2020.12595086669160743No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG1750.1091158498565906No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGGC100.0070853494145.000025
CGGTACC203.8600742E-4108.750015
AGCGAAA2050.091.951221
GCGAAAG2150.087.6744162
ATCCGAC259.372276E-487.0145
CCGGTAC259.372276E-487.04
CGAAAGC2200.085.6818163
GAGCAAA4650.077.956991
AACGCTC300.001932769172.58
GGACTAT350.00356104662.1428576
CCCGGTA350.00356104662.1428573
AGCAAAA5850.059.487182
GAGCGAA1051.0986696E-955.2380941
CAACGCT400.0060416654.3750047
CTAACCG400.0060416654.3750046
GCAAAAG6950.050.0719453
GCAACGC450.00962449648.3333326
CCTCGAT450.00962449648.3333328
GCCCGGT450.00962449648.3333322
CAAAAGC8500.040.0882344
Produced by FastQC (version 0.11.2)