FastQCFastQC Report
Mon 9 Oct 2017
000000000-B4Y2B_l01n01_3021_d-2_nw.3510000009fdd1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y2B_l01n01_3021_d-2_nw.3510000009fdd1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72768
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGCAGGATATCAAAATAGGGGTGGCGAAGGAACTCGTGTTGCAAGCCA1760.2418645558487247No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT1060.14566842568161828No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACG100.0070774495145.01
TCGAACC100.0070774495145.09
AGCGAAA800.0135.93751
GCGAAAG1050.0103.571432
CGAAAGC1250.087.03
GAGCAAA2150.070.813951
GCAAAAG2350.064.787233
GAACTAC451.18645985E-464.44445145
TATACAG451.18645985E-464.444455
TATAATA350.003553228562.1428572
AGCAAAA2650.057.4528272
CAAAAGC2700.053.70374
GGATATC553.1991696E-452.7272727
CAGCTCG604.914703E-448.3333329
AAAAGCA3350.045.447765
TACAGCT700.001050187741.428577
ATACAGC700.001050187741.428576
GAAAGCA2650.041.0377354
ATATAAT907.4141404E-540.277781
ATATCAA750.001474353338.6666649
Produced by FastQC (version 0.11.2)