FastQCFastQC Report
Mon 9 Oct 2017
000000000-B4Y2B_l01n01_3016_d8_nw.3510000009fd17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y2B_l01n01_3016_d8_nw.3510000009fd17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18881
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATACTGGGAAGGGAGACACGTCAGATCCCCAGAGAGGTTTAGGGGGGAC980.5190403050685874No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA700.37074307504899107No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG620.32837243790053494No Hit
GAGCAAAAGCAGGGGAAGTGGGAGAGGGAAAAGGAGAAGGAAGCTTCCCT580.3071871193263069No Hit
CCCAATATCACCCCCACCATGCAGGTCCTTCTCCAAGGAGTTCACCACGG540.2860018007520788No Hit
GAGCAAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCTATTCA500.26481648217785075No Hit
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT480.2542238228907367No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG430.22774217467295163No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT380.20126052645516654No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG310.16418621895026747No Hit
AGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGTCTTC300.15888988930671044No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA300.15888988930671044No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT260.13770457073248238No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC250.13240824108892538No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT210.1112229225146973No Hit
ATACTGGGAAGGGAGACACGTCAGATCCCCAGAGAGGTTTAGGGGGGACA200.10592659287114028No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA200.10592659287114028No Hit
GTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACC190.10063026322758327No Hit
TCCCAGTATCACCCCCACCATGCAGGTCCTTCTCCAAGGAGTTCACCACG190.10063026322758327No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCATG100.0070361574145.000027
TAGAAAC100.0070361574145.000022
CTTGACT100.0070361574145.000022
CATACTG100.0070361574145.000025
TACTCTC100.0070361574145.000022
GCAGGTA100.0070361574145.000029
GAGCAAA400.0145.000021
CCTATCC100.0070361574145.000023
GCGAAAG301.05990694E-7120.8333363
AGCGAAA301.05990694E-7120.8333362
ATACTGC203.8068392E-4108.750016
CGAAAGC352.657198E-7103.5714344
GAGCGAA352.657198E-7103.5714341
TATAGAC259.2434953E-487.02
AGCAAAA809.094947E-1172.500012
CAGCTCG406.495185E-572.500019
TAGACCT300.00190630972.54
ATAGACC300.00190630972.53
GTAGAAA300.00190630972.51
AAACCAT300.00190630972.56
Produced by FastQC (version 0.11.2)