FastQCFastQC Report
Mon 9 Oct 2017
000000000-B4Y2B_l01n01_3013_d8_nw.3510000009fbfd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y2B_l01n01_3013_d8_nw.3510000009fbfd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89998
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA4510.501122247161048No Hit
GAGCAAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCTATTCA3890.4322318273739416No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG2470.2744505433454077No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG2360.26222804951221135No Hit
GAGCAAAAGCAGGCATATCATCTACGAAGGAATGGCAGTGTTGGGGATTT1930.21444920998244404No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT1920.2133380741794262No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA1840.20444898775528347No Hit
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT1730.19222649392208715No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG1390.15444787661948045No Hit
GAGCAAAAGCAGGGGATGCTGCAGGCAGAGAGAGAAGCAGGCTCCCCACT1180.13111402475610567No Hit
GAGCAAAAGCAGGGGAAGTGGGAGAGGGAAAAGGAGAAGGAAGCTTCCCT1170.13000288895308784No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC1160.12889175315007No Hit
GCCGTTACACCTTTGTTCGAGTCATGATTGGGCCATGAACTTGTCTTGGG1120.12444720993799861No Hit
GTATATATGTATGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAA1120.12444720993799861No Hit
TACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCTGTGGCA1100.12222493833196293No Hit
ATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATG1050.1166692593168737No Hit
CCCCAACTGCATTTTTGACATCCTCATAAGTATGTCCTGGAAGAGAAGGT1020.11333585190782018No Hit
GCAAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCTATTCAGC930.10333562968065955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA4450.0102.640441
AGCAAAA4600.099.293482
GCAAAAG5450.083.8073353
CAAAAGC5950.077.98324
GTCTACT350.003555963762.1428572
GCGTCTG350.003555963762.1428579
AAAAGCA7500.061.866675
CGAAAGC954.474714E-1061.0526354
AAAGCAG8650.059.508676
GAGCGAA1051.0895747E-955.2380941
AGCGAAA1051.0895747E-955.2380942
GGTCTAC400.006033070454.3751
GCGAAAG1101.6461854E-952.7272763
AGCAGGT2750.052.7272728
TTACATC1056.812843E-848.3333324
TAGAAAC951.9013241E-645.7894743
ATTAAGC657.3008955E-444.6153874
AAGCAGG12100.042.541327
TACATCA1201.9467916E-742.2916685
ACAGTGG907.4281874E-540.277788
Produced by FastQC (version 0.11.2)