FastQCFastQC Report
Mon 9 Oct 2017
000000000-B4Y2B_l01n01_3011_d8_nw.3510000009f9af.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y2B_l01n01_3011_d8_nw.3510000009f9af.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147649
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG20301.374882322264289No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA12870.8716618466769163No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA5790.39214623871478976No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT4980.3372864022106482No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC4620.31290425265325195No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG4430.300035895942404No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG4210.28513569343510625No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC3920.26549451740275926No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT3790.2566898522848106No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT3740.2533034426240611No Hit
TCCACCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCAT3680.24923975103116175No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC3670.24856246909901186No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT3620.24517605943826237No Hit
GTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTTATC3490.2363713943203137No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT3440.23298498465956421No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG3370.22824401113451498No Hit
GAGCAAAAGCAGGGGAAGTGGGAGAGGGAAAAGGAGAAGGAAGCTTCCCT3220.2180847821522665No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT3040.2058937073735684No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCT2870.19437991452702016No Hit
CCCCAACTGCATTTTTGACATCCTCATAAGTATGTCCTGGAAGAGAAGGT2530.1713523288339237No Hit
GCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAAC2500.16932048303747402No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC2120.14358376961577796No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA2060.13952007802287858No Hit
GAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA2000.1354563864299792No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT1980.1341018225656794No Hit
AGTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTTAT1950.13206997676922974No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC1840.12461987551558087No Hit
GGTGCAAGATCCCAATGATATTTGCTGCAATGACGAGAGGATCACTTGAA1800.12191074778698129No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATG1790.1212334658548314No Hit
GTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTC1710.11581521039763223No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT1710.11581521039763223No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC1690.11446064653333242No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA1660.11242880073688273No Hit
GGTCCAATCGCACGCAAAGAGGGCCTATTATCTTTTGCCTAGGCATGAGC1660.11242880073688273No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT1630.11039695494043306No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA1620.10971967300828316No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC1600.10836510914398337No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG1590.10768782721183347No Hit
GTATATTTTCAGAGACTCGAACCGTGTTACCATTCCATTCAAGTCCTCCG1590.10768782721183347No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC1590.10768782721183347No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC1590.10768782721183347No Hit
GACTCGAACCGTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAA1580.10701054527968357No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC1560.10565598141538378No Hit
GCCGTTACACCTTTGTTCGAGTCATGATTGGGCCATGAACTTGTCTTGGG1550.10497869948323389No Hit
ATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCCAAGGAGGTGT1540.10430141755108399No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT1530.10362413561893409No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT1510.1022695717546343No Hit
TTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCCC1500.10159228982248442No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG1490.10091500789033452No Hit
GTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGT1480.10023772595818461No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTATG100.0070847827145.01
ACGTGAG100.0070847827145.05
TCACGTC100.0070847827145.03
GAGCGAA1350.0112.777781
GAGCAAA9400.0108.751
AGCAAAA11450.088.6462862
GCAGGTA3200.086.093759
AGCGAAA1750.082.857152
GCAAAAG12350.082.186233
GCAGGGT5150.078.8349469
GCGAAAG1900.076.315783
AAGCAGG15350.075.570037
AGCAGGT5150.073.203888
CCTACTA1000.072.55
CAAAAGC14100.071.471634
AAAGCAG16400.070.2896356
CACCTAC1150.069.347833
CGAAAGC2150.067.441864
CCACCTA1200.066.4583362
AAAAGCA15250.066.081965
Produced by FastQC (version 0.11.2)