FastQCFastQC Report
Mon 9 Oct 2017
000000000-B4Y2B_l01n01_3010_d8_nw.3510000009fa27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y2B_l01n01_3010_d8_nw.3510000009fa27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151726
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG12050.7941947985183818No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA6390.4211539222018639No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT6090.40138143759144773No Hit
GAGCAAAAGCAGGGGAAGTGGGAGAGGGAAAAGGAGAAGGAAGCTTCCCT5210.34338214940089373No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG4890.32229149914978317No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC4100.270223956342354No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA3460.22804265584013286No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT2950.19442943200242543No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA2880.18981585225999498No Hit
CCCCAACTGCATTTTTGACATCCTCATAAGTATGTCCTGGAAGAGAAGGT2790.18388410687687012No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC2210.14565730329673227No Hit
GCCGTTACACCTTTGTTCGAGTCATGATTGGGCCATGAACTTGTCTTGGG2080.1370892266322186No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG1870.12324848740492732No Hit
GGCAAGTGCACCAGTTGAATAGCTTAGTGACACCTCCTTGGCCCCATGGA1810.11929399048284407No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG1790.11797582484214966No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC1770.11665765920145525No Hit
GCACTGCAGGGGGGGGTGCTCTGGGGGAATGAAAGAGGGAGGCCCCCGCA1720.11336224509971922No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT1650.1087486653572888No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT1590.10479416843520556No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT1580.10413508561485836No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC1550.10215783715381674No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA1530.10083967151312233No Hit
GAGCAAAAGCAGGCAAGTGGGAGTTCAGAGAGGAGAACAGTTGCTGAGAG1520.10018058869277514No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTACG100.007084977145.01
TACGGTG100.007084977145.04
GAGCGAA1850.0109.729721
GAGCAAA7400.0105.810811
AGCAAAA7900.097.278482
TACACCG259.3712937E-487.05
GCAAAAG10000.076.1253
AGCGAAA2700.075.185192
CAAAAGC10450.072.846894
GGTACGG300.001932567372.52
GCAGGTA1900.072.59
GTACGGT300.001932567372.53
CGAAAGC2900.070.04
GCGAAAG2900.070.03
AAAGCAG14500.067.06
CTAGTGC651.3900717E-766.923079
AAAAGCA11650.065.965675
ATAGACC451.1906088E-464.444443
TAGACCC502.0047878E-458.04
AAGCAGG18050.055.02777
Produced by FastQC (version 0.11.2)