FastQCFastQC Report
Mon 9 Oct 2017
000000000-B4Y2B_l01n01_3008_d8_nw.3510000009f9c8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y2B_l01n01_3008_d8_nw.3510000009f9c8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188370
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG15430.8191325582629929No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA9300.4937091893613633No Hit
GATACTGGGAAGGGAGACACGTCAGATCCCCAGAGAGGTTTAGGGGGGAC6740.35780644476296647No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG5200.27605244996549344No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC4670.24791633487285658No Hit
GAGCAAAAGCAGGGGAAGTGGGAGAGGGAAAAGGAGAAGGAAGCTTCCCT4490.23836067314328183No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT4080.21659499920369485No Hit
CCCAATATCACCCCCACCATGCAGGTCCTTCTCCAAGGAGTTCACCACGG4050.21500238891543239No Hit
TCTGATAGGCCTGCAAATTTTCAAGAAGGTCATCTTTCAGACCAGCACTG3440.18261931305409565No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT3260.1730636513245209No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG3240.17200191113234592No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG3140.16669321017147104No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC3000.15926102882624624No Hit
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT2890.15342145776928384No Hit
CCCCAACTGCATTTTTGACATCCTCATAAGTATGTCCTGGAAGAGAAGGT2880.15289058767319635No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC2690.1428040558475341No Hit
GCCGTTACACCTTTGTTCGAGTCATGATTGGGCCATGAACTTGTCTTGGG2570.13643361469448426No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT2540.1348410044062218No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT2530.1343101343101343No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT2510.13324839411795936No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA2320.12316186229229707No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC2060.1093592397940224No Hit
GCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAAC2050.10882836969793493No Hit
GGCAAGTGCACCAGTTGAATAGCTTAGTGACACCTCCTTGGCCCCATGGA1990.10564314912140998No Hit
GGTCCAATCGCACGCAAAGAGGGCCTATTATCTTTTGCCTAGGCATGAGC1970.10458140892923501No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA9550.0120.706811
AGCAAAA12100.095.26862
GAGCGAA2400.084.5833361
GCAAAAG14800.078.378373
CAAAAGC15850.072.72874
CCTACTA702.7957867E-972.55
CTACACT554.0488285E-665.909094
AAAAGCA17400.065.833335
AAAGCAG20400.065.747556
AGCGAAA3000.065.252
GCAGGGT7750.062.6774189
GGTCGTT350.003562011562.1428577
GCGAAAG3150.062.1428573
CGAAAGC3200.061.1718754
CTAGTGC1007.1486284E-1058.09
GCAGGTA3650.057.602749
AAGCAGG23400.057.3183757
GATATAT701.6906888E-551.7857131
GTAGAAA2200.049.4318161
CACCTAC1056.866503E-848.3333323
Produced by FastQC (version 0.11.2)