FastQCFastQC Report
Mon 9 Oct 2017
000000000-B4Y2B_l01n01_3007_d8_nw.3510000009fc32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y2B_l01n01_3007_d8_nw.3510000009fc32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75725
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG6520.8610102344007924No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA4130.5453945196434467No Hit
GATACTGGGAAGGGAGACACGTCAGATCCCCAGAGAGGTTTAGGGGGGAC3440.45427533839551004No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG2490.3288213931990756No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC2020.26675470452294486No Hit
CCCAATATCACCCCCACCATGCAGGTCCTTCTCCAAGGAGTTCACCACGG2010.26543413667877186No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT1910.25222845823704193No Hit
GAGCAAAAGCAGGGGAAGTGGGAGAGGGAAAAGGAGAAGGAAGCTTCCCT1840.24298448332783096No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT1460.1928029052492572No Hit
CCCCAACTGCATTTTTGACATCCTCATAAGTATGTCCTGGAAGAGAAGGT1400.18487949818421923No Hit
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT1350.17827665896335423No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC1260.1663915483657973No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA1180.15582700561241333No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA1150.15186530207989435No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT1150.15186530207989435No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG1150.15186530207989435No Hit
GGTCCAATCGCACGCAAAGAGGGCCTATTATCTTTTGCCTAGGCATGAGC1050.1386596236381644No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG1030.13601848794981844No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC950.12545394519643446No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT920.12149224166391548No Hit
GAGCAAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCTATTCA910.12017167381974249No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC900.1188511059755695No Hit
GAGCAAAAGCAGGCAAGTGGGAGTTCAGAGAGGAGAACAGTTGCTGAGAG860.11356883459887751No Hit
GGCAAGTGCACCAGTTGAATAGCTTAGTGACACCTCCTTGGCCCCATGGA850.11224826675470452No Hit
GCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAAC790.10432485968966657No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT780.10300429184549358No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG780.10300429184549358No Hit
GTATATTTTCAGAGACTCGAACCGTGTTACCATTCCATTCAAGTCCTCCG770.10168372400132057No Hit
AGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGTCTTC770.10168372400132057No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT760.10036315615714758No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAAGG100.007078013145.06
AGTACGT100.007078013145.05
TAGTACG100.007078013145.04
GACTACT100.007078013145.04
GAGCAAA3650.0117.191791
AGCAAAA4400.095.5681842
GCAGGTA1600.095.156259
TAGACAG259.353025E-487.05
CCTACTA259.353025E-487.04
GCAAAAG4900.085.816323
CAAAAGC5300.079.339624
CCAATAT406.623512E-572.52
AGCAGGT2850.071.228078
AAAAGCA6150.069.552845
GAGCGAA1050.069.0476151
AAAGCAG7600.065.822376
GTAGAAA902.764864E-1064.444441
AGCGAAA1200.060.4166682
GCAGGGT2300.059.8913089
AAGCAGG8500.059.705887
Produced by FastQC (version 0.11.2)