FastQCFastQC Report
Mon 9 Oct 2017
000000000-B4Y2B_l01n01_3006_d8_nw.3510000009f992.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y2B_l01n01_3006_d8_nw.3510000009f992.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105218
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG6190.5883023817217586No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA4410.41912980668706873No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA3560.33834515006937976No Hit
GAGCAAAAGCAGGGGAAGTGGGAGAGGGAAAAGGAGAAGGAAGCTTCCCT3250.30888251059704613No Hit
GAGCAAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCTATTCA3100.2946263947233363No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT2780.2642133475260887No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG2550.24235396985306695No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC2180.2071888840312494No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG2150.20433766085650745No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA1760.1672717595848619No Hit
GCCGTTACACCTTTGTTCGAGTCATGATTGGGCCATGAACTTGTCTTGGG1640.15586686688589405No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT1560.14826360508658215No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT1480.14066034328727023No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT1430.1359083046627003No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC1410.1340074892128723No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG1310.12450341196373245No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC1160.11024729609002261No Hit
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT1160.11024729609002261No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC1140.10834648064019464No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG1130.10739607291528067No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT1090.1035944420156247No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTTAT100.0070819096145.06
GAGCAAA5100.0112.303921
GGCGGTT203.8563422E-4108.757
GAGCGAA900.096.6666641
AGCAAAA6700.084.402992
GCAAAAG7300.077.465763
CATACAT1050.075.9523858
CAAAAGC7750.072.967744
CTCTAGC406.6355315E-572.51
AAAAGCA8150.070.276085
AGCGAAA1250.069.62
GCAGGTA1950.066.923089
AAAGCAG10000.064.5256
GCGAAAG1400.062.1428573
TTAGACG350.00355764162.142857145
CGCTCCC350.00355764162.1428576
GGGCGGT350.00355764162.1428576
TCGCTCC350.00355764162.1428575
CGAAAGC1450.060.0000044
AAGCAGG11450.056.98697
Produced by FastQC (version 0.11.2)