FastQCFastQC Report
Mon 9 Oct 2017
000000000-B4Y2B_l01n01_3005_d5_nw.3510000009fb83.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y2B_l01n01_3005_d5_nw.3510000009fb83.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136432
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG15871.1632168406239005No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA13751.0078280755248037No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA9270.6794593643719948No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT8460.6200891286501701No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG5520.4045971619561393No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCT4480.3283687111528087No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT4450.3261698135334819No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT4320.31664125718306557No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC3470.25433915796880496No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG3000.21988976193268442No Hit
TCCACCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCAT2980.2184238301864665No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC2900.21256010320159494No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG2860.20962823970915914No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT2840.20816230796294125No Hit
GCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAAC2800.20523044447050545No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT2760.20229858097806966No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC2690.19716781986630702No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG2630.19277002462765333No Hit
GTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTTATC2580.1891051952621086No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC2560.1876392635158907No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA2470.18104257065791018No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATG2390.17517884367303857No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC2320.17004808256127596No Hit
GAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA2160.15832062859153279No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT2140.1568546968453149No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC2140.1568546968453149No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG2090.15318986747977015No Hit
GAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATAACCAT2020.1480591063680075No Hit
GCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGA2000.1465931746217896No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT1900.13926351589070013No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA1890.13853055001759118No Hit
ATATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTATGA1840.13486572065204644No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC1760.12900199366717485No Hit
GGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTT1760.12900199366717485No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT1730.126803096047848No Hit
AGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG1720.12607013017473906No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG1680.12313826668230328No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA1660.12167233493608537No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC1640.12020640318986747No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG1640.12020640318986747No Hit
CACTAGACCAAAAGCAGCTTCTGTGGTCACTGTTCCCATCCTGTTGTATA1630.11947343731675854No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA1620.11874047144364959No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT1600.11727453969743169No Hit
GCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGGCG1590.11654157382432273No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC1590.11654157382432273No Hit
GTCCTACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCAG1590.11654157382432273No Hit
GAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA1580.1158086079512138No Hit
TTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCCC1560.11434267620499589No Hit
GGGTTTCGACTTTCTCTTACTTGATCCATCATTGCCCTCTGGGCAGCTGT1530.11214377858566905No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAGTGAAC1510.11067784683945117No Hit
GTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGTAATCTCGTCA1500.10994488096634221No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA1500.10994488096634221No Hit
ACATCCACAGCACTCTGCTGTTCCTGTTGATATTCTTCCCTCATGGACTC1500.10994488096634221No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA1490.10921191509323325No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA1480.10847894922012431No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC1460.10701301747390643No Hit
AGTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTTAT1450.10628005160079747No Hit
AGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAA1440.1055470857276885No Hit
ATGATGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT1440.1055470857276885No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC1430.10481411985457957No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG1400.10261522223525273No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT1390.10188225636214379No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAGTA151.2276959E-4145.07
ATATCTA100.0070841983145.03
TATAGAG100.0070841983145.02
TCCCCCC202.1239466E-6145.09
GAGCGAA1300.0139.423081
TCGCTCC203.8588245E-4108.755
GAGCAAA8200.0105.213421
GCAGGTA3250.091.461549
TTTCGCT259.369253E-487.03
CTTTCGC259.369253E-487.02
TTCGCTC259.369253E-487.04
GCTTTCG259.369253E-487.01
AGCGAAA2100.086.3095252
GCAGGGT4100.084.878059
AGCAAAA10200.084.5833362
CGAAAGC2250.080.555554
GCGAAAG2250.080.555553
AAGCAGG13050.080.555557
GCAAAAG10900.079.1513753
AGCAGGT6300.073.6507958
Produced by FastQC (version 0.11.2)