FastQCFastQC Report
Mon 9 Oct 2017
000000000-B4Y2B_l01n01_3004_d8_nw.3510000009fbc6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y2B_l01n01_3004_d8_nw.3510000009fbc6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97223
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG7890.8115363648519383No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA4970.5111959104327165No Hit
GATACTGGGAAGGGAGACACGTCAGATCCCCAGAGAGGTTTAGGGGGGAC3800.3908540160250147No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA2590.26639786881704947No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC2480.25508367361632533No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG2440.25096942081606205No Hit
CCCAATATCACCCCCACCATGCAGGTCCTTCTCCAAGGAGTTCACCACGG2320.2386266624152721No Hit
GAGCAAAAGCAGGGGAAGTGGGAGAGGGAAAAGGAGAAGGAAGCTTCCCT2090.21496970881375804No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT2090.21496970881375804No Hit
CCCCAACTGCATTTTTGACATCCTCATAAGTATGTCCTGGAAGAGAAGGT1870.19234131841230984No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT1720.17691287041132242No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC1590.16354154881046665No Hit
GCACTGCAGGGGGGGGTGCTCTGGGGGAATGAAAGAGGGAGGCCCCCGCA1560.16045585921026917No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG1520.15634160641000586No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC1460.1501702272096109No Hit
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT1350.13885603200888677No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT1340.13782746880882096No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT1200.12342758400789937No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT1190.12239902080783352No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG1100.11314195200724107No Hit
GAGCAAAAGCAGGCAAGTGGGAGTTCAGAGAGGAGAACAGTTGCTGAGAG1070.1100562624070436No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA1020.10491344640671448No Hit
GCCGTTACACCTTTGTTCGAGTCATGATTGGGCCATGAACTTGTCTTGGG1010.10388488320664863No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT980.10079919360645115No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCCG100.007081087145.08
GAGCAAA4650.0115.376341
GAGCGAA1450.0100.01
AGCAAAA5650.093.672572
GCAAAAG6750.079.481483
TATAATA300.001930470772.52
GCTTTCG406.632993E-572.51
AGCGAAA2100.069.0476152
CGAAAGC2200.065.9090964
GCGAAAG2200.065.9090963
CAAAAGC7950.065.660384
TTTCGCT451.1884641E-464.444443
CTTTCGC451.1884641E-464.444442
GCAGGTA1750.062.1428579
AGCAGGT3500.062.1428578
AAAGCAG10800.061.7592546
GCAGGGT3150.059.841279
AAAAGCA8900.058.6516885
TTACACA502.0011872E-458.04
AAGCAGG11600.057.57
Produced by FastQC (version 0.11.2)