FastQCFastQC Report
Mon 9 Oct 2017
000000000-B4Y2B_l01n01_3003_d-2_nw.3510000009f8e3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y2B_l01n01_3003_d-2_nw.3510000009f8e3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124211
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT4370.35182069220922463No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA3060.24635499271401085No Hit
AGCAAAAGCAGGCAGGTGTTGGCTGGCAGTGGGACCAGTGGACTGAGTCA2440.1964399288307799No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG1920.15457568170290875No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG1800.14491470159647696No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA1780.14330453824540498No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA1640.13203339478790124No Hit
GAGTGATAAAGTGGGATAAAGCAGTCAGGAAGCAGTAAGAATGAGGTGTG1440.11593176127718156No Hit
TTCTTATACATGCCCTTAAGGACCATCTGAATGTGAAGACTCGAGAATTA1320.10627078117074976No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1350.091.2962951
CGAAAGC1500.082.166673
GCGAAAG1500.082.166672
GAGCAAA2550.065.392161
TACATAC902.7830538E-1064.444446
CATACAT1051.0950316E-955.2380948
TCCGGCA400.006038466554.374996145
ATAGACA1850.050.945953
TATAGAC1750.049.7142832
TGCATTG1351.8735591E-1048.3333365
TATAGGT450.00961942448.3333324
AGCAAAA3350.047.611942
TTGTCTC1551.4551915E-1146.7741939
GCATTGT1453.783498E-1045.0000046
CGTCTGA657.311697E-444.615387145
GCAAAAG3750.044.4666673
TAGACAT2050.042.4390264
CATTGTC1557.2941475E-1042.0967757
CTACAGT700.001053067341.428574
CAAAAGC4100.040.670734
Produced by FastQC (version 0.11.2)