FastQCFastQC Report
Mon 9 Oct 2017
000000000-B4Y2B_l01n01_3002_d8_nw.3510000009fd0a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y2B_l01n01_3002_d8_nw.3510000009fd0a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32518
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA1490.45820776185497264No Hit
GAGCAAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCTATTCA1150.3536502859954487No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG830.2552432498923673No Hit
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT650.19988929208438402No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG530.16298665354572853No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA520.15991143366750724No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT490.15068577403284333No Hit
GAGCAAAAGCAGGGGAAGTGGGAGAGGGAAAAGGAGAAGGAAGCTTCCCT440.1353096746417369No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC380.11685835537240913No Hit
GCACTGCAGGGGGGGGTGCTCTGGGGGAATGAAAGAGGGAGGCCCCCGCA350.10763269573774525No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG330.10148225598130266No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAAGT100.007059553145.04
GAGCAAA950.099.210531
AGCGAAA651.5024852E-978.076922
TATACAT300.001918875572.54
AGCAAAA1350.069.814822
GAGCGAA754.660251E-967.6666641
CAAAAGC1550.065.483874
AAAGCAG2500.060.8999986
AAAAGCA1750.057.9999965
TACATAC400.005998552354.3756
TTTGCGG400.005998552354.375145
CGAAAGC953.014975E-853.4210554
GCAAAAG1850.050.9459423
GCGAAAG1056.6334906E-848.3333323
CTCTTAA450.009556076548.3333329
TTTTGCT450.009556076548.3333323
AGCAGGT1203.43789E-948.3333328
AAGCAGG3550.042.8873257
GCAGGTG1156.932045E-637.8260889
GAAAGCA1354.776957E-737.5925945
Produced by FastQC (version 0.11.2)