FastQCFastQC Report
Mon 9 Oct 2017
000000000-B4Y2B_l01n01_3002_d-2_nw.3510000009fe5a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y2B_l01n01_3002_d-2_nw.3510000009fe5a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164058
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT6560.3998585865974229No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA5220.3181801557985591No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG4930.30050348047641684No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACCTCTTAACAATA2190.13348937570859087No Hit
GGTCTATACTACCCAAAATCTACATATTTGATGTAATACCTATCAAAATA1930.11764132197149789No Hit
ATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGAGT1690.10301234929110435No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC2600.094.807693
GCGAAAG2700.091.2962952
AGCGAAA2850.086.491231
GTAAAGA350.003561192262.1428572
GGGATAG753.7417158E-758.05
GATAGAC701.6897235E-551.7857137
GTCCTAT657.3185936E-444.6153871
GAAAGCA5800.042.54
CAGCTCG2050.042.4390269
ATTATAC700.001054057641.428573
TTACTCA1807.094059E-1140.277784
TGGGATA907.455079E-540.277784
ATATGAC1105.237416E-639.5454523
ATGACAG1157.1063023E-637.8260885
TAGGTAC2201.4551915E-1136.25145
GTATGGG1001.3863854E-436.251
TACTCAG1655.964648E-835.1515165
GAGCAAA2700.034.907411
ATGGGAT1051.8469869E-434.5238083
CCTACAG1051.8469869E-434.5238082
Produced by FastQC (version 0.11.2)