FastQCFastQC Report
Mon 9 Oct 2017
000000000-B4Y2B_l01n01_3001_d5_nw.3510000009fac7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y2B_l01n01_3001_d5_nw.3510000009fac7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34739
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA5811.6724718615964764No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA4491.2924954661907366No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT2940.8463110624946026No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG2850.8204035809896658No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG2230.6419298195112122No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATG1840.5296640663231527No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT1630.4692132761449667No Hit
GAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA1310.37709778634963587No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG1220.35119030484469904No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA1040.2993753418348254No Hit
AGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG1010.29073951466651315No Hit
ATATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTATGA930.26771064221768043No Hit
ATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTATGACA910.26195342410547223No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA840.2418031607127436No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG820.23604594260053544No Hit
GCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGA800.23028872448832724No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT770.22165289732001495No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT720.2072598520394945No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCT700.20150263392728635No Hit
GCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAAC670.19286680675897405No Hit
ATGATGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT620.17847376147845362No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG590.16983793431014135No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG580.16695932525403726No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC570.16408071619793316No Hit
GAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA570.16408071619793316No Hit
GCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGGCG500.14393045280520453No Hit
AGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGGC500.14393045280520453No Hit
GAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG500.14393045280520453No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA490.14105184374910043No Hit
AGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAA490.14105184374910043No Hit
AAATGGGAATGGGGACCCGAACAACATGGATAGAGCAGTTAAACTATACA490.14105184374910043No Hit
GAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATAACCAT480.13817323469299633No Hit
ACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAATA480.13817323469299633No Hit
AGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGGC480.13817323469299633No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG470.13529462563689224No Hit
AGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAA450.12953740752468407No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC450.12953740752468407No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC430.1237801894124759No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC430.1237801894124759No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC420.1209015803563718No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT410.11802297130026772No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT400.11514436224416362No Hit
CCATATGTACCAGAAGTGCTGCAATCTATTCGAGAAATTTTTCCCTAGCA390.11226575318805954No Hit
TCCCAGTGCTGGGAAGGACCCTAAGAAAACAGGAGGACCCATATATAGAA380.10938714413195544No Hit
TCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGAT380.10938714413195544No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT370.10650853507585135No Hit
GCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGTAG360.10362992601974726No Hit
CATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAG360.10362992601974726No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG360.10362992601974726No Hit
TTCCACAAGAGGGGTCCAGATTGCTTCAAATGAGAATGTGGAAACCATGG350.10075131696364317No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC350.10075131696364317No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC350.10075131696364317No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC350.10075131696364317No Hit
ATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAGGGAAAAATTT350.10075131696364317No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA350.10075131696364317No Hit
GGATACATATGCAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAA350.10075131696364317No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA350.10075131696364317No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTATA100.007061603145.02
GTTATAC100.007061603145.03
GAGCGAA404.6784407E-9108.751
AGCAGGC950.0106.842118
GCAGGTA1250.0104.49
GAGCAAA4350.0101.6666641
GCAAAAG4550.097.197813
GCAGGGT1200.096.6666649
CAAAAGC4600.096.14134
AAGCAGG5150.095.728157
AAAGCAG5250.092.52386
AGCAAAA4800.092.1354142
AGTAGAA405.99317E-790.6251
AAAAGCA5300.084.8113255
GCAGGCA1200.084.5833369
CAAAAGT1150.081.95652145
GTAGAAA451.2078945E-680.555562
AGCAGGG2000.079.758
AGCGAAA651.5061232E-978.076931
AGCAGGT3000.077.3333368
Produced by FastQC (version 0.11.2)