FastQCFastQC Report
Mon 9 Oct 2017
000000000-B4Y2B_l01n01_3001_d-2_nw.3510000009f91b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y2B_l01n01_3001_d-2_nw.3510000009f91b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140388
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA5160.36755278228908456No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG3760.2678291591873949No Hit
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT2260.16098242014987035No Hit
ATTATATATTTAGGGTTTGTTGTCGATGCAAGTATTTGTTAATTAAGTCA1620.11539447816052653No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTCG100.0070844158145.01
CGAAAGC2200.085.6818163
GCGAAAG2150.084.302332
AGCGAAA2200.082.386361
GAGCAAA1850.070.5405351
TTTAACG451.1902989E-464.444458
TTAACGT451.1902989E-464.444459
GGAGCGA502.0042685E-458.01
TAGGTAC1007.1122486E-1058.0145
GGCCCGC400.006040096754.3756
GGTATAG400.006040096754.3751
TACATAC1650.052.7272766
GAAAGCA3900.050.192314
CATACAT1750.049.7142878
ATAGACA901.3153858E-648.3333363
GCCTAAC450.00962201148.3333361
CAGCTCG1509.094947E-1248.3333369
ATACAGC1602.0008883E-1145.31256
GGTCTAT1002.7172955E-643.51
TATACAG1903.6379788E-1241.9736865
Produced by FastQC (version 0.11.2)