FastQCFastQC Report
Tue 31 Oct 2017
000000000-B4WPY_l01n03.333000000a3aaf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4WPY_l01n03.333000000a3aaf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17831683
Sequences flagged as poor quality0
Sequence length251
%GC54

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAA14427098.09070573989006No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAG9554625.358226702437453No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA8872214.975531473950047No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGCGTCAGTTACAGACCAGAG7510104.211660783785804No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAC4414672.4757449983829347No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG3205301.7975308331804687No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG2678221.5019446005180779No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGG2250951.2623317720486618No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA2125501.19197946710919No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAG1879101.0537984552551771No Hit
CCTATTTGCTCCCCACGCTTTCGTGCTTCAGTGTCAGTATCCAGACCAGA1805371.0124507036155814No Hit
CCTATTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTACAGGCCAGGC1720350.9647715249312138No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCACTGTCCAGTA1574040.8827209411472825No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1516060.8502057825949464No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG1487310.8340827952134412No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA1405730.7883327670192432No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAG1131030.6342811275862184No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACGGCCCAGCA1105550.6199919547694965No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA992950.5568459241901058No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG989320.5548102217833281No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAG957910.537195507569308No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAG903840.5068730753008563No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTGATCGTCCAGTA819470.45955841633120104No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA738810.41432432373321126No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCACCCCGGAT724460.4062768500314861No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGTCCAGAA720160.40386541191877406No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA646340.36246718831867974No Hit
CCTGTTTGATACCCGCACTTTCGAGCATCAGCGTCAGTTACGGTCCAGCA608260.3411119410321505No Hit
CCTGTTTGCTCCCCATGCTTTCGCGCTTCAGCGTCAGTATCTGTCCAGTG601900.33754525582358097No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA583230.3270751280179218No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTT573290.32150078038062924No Hit
CCTATTTGCTCCCCACGCTTTCGTACTTCAGCGTCAGTTTCAGACCAGGC568830.31899961433814183No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTATCGTCCAGTA546870.30668445597647737No Hit
CCTATTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGCAACAGTCCAGGC540190.30293831490835726No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA513270.28784159072365745No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTATCGTCCAGAA487280.2732664101307768No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA478100.26811827015991707No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA457950.25681815900383603No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTTTCCAGCCAGAT450750.25278040216394604No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA441150.24739672637742607No Hit
CCTGTTCGCTCCCCTAGCTTTCGCACCTCAGCGTCAGTTACGGCCCAGAA423220.2373415902469778No Hit
CCTGTTCGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG421220.23621999112478617No Hit
CCTGTTTGCTCCCCACGCTTTCGTACCTCAGTGTCAGTTACAGTCCAGAA400060.22435347241199832No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCACCCCGGAC390370.21891932466497974No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAACGTCAGTTACCGTCCAGTA388590.21792110144622914No Hit
CCTGTTTGCTCCCCACACTTTCGTACCTCAGTGTCAGTTACTGTCCAGAA377760.21184764219956131No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAA371640.2084155488856548No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA370860.20797812522800008No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA370670.20787157331139186No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTTGAGCGCCGGTA364660.20450116794920592No Hit
CCTGTTCGCTCCCCCGGCTTTCGCGCCTCAGCGTCAGTGTCGGCCCAGCA364070.20417029620815938No Hit
CCTGTTTGATACCCGCACTTTCGAGCATCAGCGTCAGTTACGGTCCGGCA354130.1985959485708668No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTTACAGACCAGAG348090.195208719221848No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGTA343360.1925561372978647No Hit
CCTGTTCGCTACCCATGCTTTCGCTCCTCAGCGTCAGTTACTACCCAGAG335900.1883725725720898No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACGGCTTAGTG315420.17688739756084718No Hit
CCTGTTCGCTACCCATGCTTTCGCTCCTCAGCGTCAGTTACTGCCCAGAG313070.175569518592272No Hit
CCTGTTTGATACCCGCACTTTCGAGCATCAGCGTCAGTTACGGTCCAGTA306920.1721206012915326No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTCCAGAA304720.17088684225712178No Hit
CCTGTTCGCTACCCATGCTTTCGCGCCTCAGCGTCAGTTGCAGACCAGAG297740.16697246132067287No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCGGCCCAGTA286820.1608485301135064No Hit
CCTATTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTAACAGTCCAGGC274780.1540965033979126No Hit
CCTATTTGCTCCCCACGCTTTCGTGCTTCAGTGTCAGAATCCAGACCAGA271560.152290728811184No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAGTTGGGCGCCGGTG265990.14916707525588022No Hit
CCTGTTTGCTCCCCACGCTTTCGTTCCTCAGTGTCAGTTACAGTCCAGAA252560.1416355371503632No Hit
CCTATTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTACAGACTAGCA244510.13712110068354177No Hit
CCTGTTCGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAG242870.1362013894033446No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTCATCGTCCAGCA231240.12967929050780008No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCAGTGGCGGCCCAGCA224360.12582098952746076No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTAGATGCCCAGAG207680.11646685284838229No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCATCAGCGTCAGTAATGTTCCAGCA206550.11583314934434401No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGGCCAGAG206120.11559200553307278No Hit
CCTGTTCGCTACCCACGCTTTCGAGCCTCAGCGTCAGTCACAGACCAGAG202200.11339367125357713No Hit
CCTGTTCGCTCCCCCAGCTTTCGCACCTCAGCGTCAGTGACGGCCCAGAA201000.11272071178026212No Hit
CCCTTTCGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGTTAATGTCCAGGA199690.11198606435522661No Hit
CCTGTTTGCTCCCCACGCTTTCGAACCTCAGCGTCAGTTACAGACCAGAG198630.111391616820465No Hit
CCTGTTTGATCCCCGCACTTTCGTGCATCAGCGTCAATGCCTGCTTCGTG198560.1113523608511883No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGTA195530.10965313818106794No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGCA193740.1086493069667064No Hit
CCTATTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTACAGACCAGGT193600.10857079502815299No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTTAATGCCCAGAG191050.1071407561473586No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT189220.10611449295055324No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGTCCAGAG187720.1052732936089095No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG187120.104936813872252No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG186550.10461715812242736No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGCA183420.10286185549619742No Hit
CCCATTTGCTACCCTGGCTTTCGTTCCTCAGTGTCAATTACGGTCCAGTA183340.10281699153130974No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGCA183290.10278895155325496No Hit
CCTGTTTGCTCCCCATGCTTTCGCGCTTCAGTGTCAGTATTCGTCCAGTG182250.10220572000971528No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCAGACCGGAGCCGGTA181180.10160566447934276No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA179000.10038312143615384No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTGC9489500.0244.73013
CTGTTCG3740750.0244.570892
TTTGCTC8862950.0244.462255
TGTTCGA464700.0244.156333
ATTTGCT1389200.0243.994644
GTTTGCT9549800.0243.968554
CTGTTTG10519500.0243.831912
TGTTTGA1015500.0243.443773
TGTTCGC3303850.0243.146013
CCTGTTC3732050.0242.979451
CCTGTTT10547300.0242.063891
CTATTTG1374200.0241.398532
CCTATTT1374100.0240.957281
TTTGCTA2134500.0240.948145
TATTTGC1376700.0240.097053
TGCTACC2173350.0238.873057
GTTCGCT3408650.0238.804184
GCTCCCC10331600.0238.604328
TTTGATC461850.0238.31595
CTCCCCA9554300.0238.263499
Produced by FastQC (version 0.11.2)