FastQCFastQC Report
Tue 31 Oct 2017
000000000-B4WPY_l01n02.332000000a3aa2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4WPY_l01n02.332000000a3aa2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17831683
Sequences flagged as poor quality0
Sequence length12
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGTCTAGTTG4957612.7802255120843054No Hit
GGACAACGCTTG4702452.637131896075093No Hit
ACTTGCCCTCAC4649972.6077011351087838No Hit
CTACACAAGCAC4594532.5766104074416307No Hit
GGAATAAACGTG4383942.4585116278704593No Hit
CTACTACAGGTG4078282.2870976340259075No Hit
CATGAGTGCTAC4053872.2734085167395586No Hit
CGTGAGGCATTG4005802.246450881837682No Hit
CGTTTCCGTTTC3999902.2431421644272165No Hit
TCTTAGACGACG3943492.2115074611858008No Hit
TAGTACCCGAGG3910392.1929449957135283No Hit
TGATAGTGAGGA3804182.13338247432954No Hit
CGCTTATCGAGA3794952.1282062943806257No Hit
ATGCCAACCAAC3706632.0786764771446418No Hit
CCACGTGTGGAA3587082.0116328896156355No Hit
ATCGCCACGTAA3550551.9911468816488045No Hit
CGCATTTATACG3469811.945867925085927No Hit
GTTATCCGTACA3460021.9403776973827989No Hit
CATGACCTGGAG3353801.8808095680031995No Hit
CAAGACACGACC3331831.8684888016459245No Hit
GAGTAGCTCGTG3194011.7911994061356968No Hit
TGTTGACACTAC3169351.7773700889590733No Hit
GTTAAGCCCGAT3155571.769642271007173No Hit
TGGAAGATGAGT3061711.7170056242027183No Hit
ATCATCTGGGTT3002031.683537106396519No Hit
GTGAACTTGGGC2983181.6729660346698625No Hit
GGAAGCTTGTCT2958691.6592320534186258No Hit
CGACAGCTGACA2949811.6542521533160948No Hit
GATACAGGTGAA2895691.6239016810695883No Hit
AGGTCGTATGCG2749971.5421819690267038No Hit
CCTCCTCTTTAT2723021.527068420855171No Hit
TTATCCGAGGTT2657071.490083689800901No Hit
TCGGACCTAGGA2633511.4768712521414833No Hit
ACAATTTCCGAC2633121.4766525403126558No Hit
CTGATGGGCGTA2410731.351936325920554No Hit
GTACGAATCCTG2399321.3455376029284505No Hit
TCTAGGAGTTTC2353091.3196118392189902No Hit
AAGATTCGGCGC2031491.1392587003705708No Hit
TCAGTACGAGGC1976461.1083979005234672No Hit
GCCTAGTTGATT1965131.1020440414962513No Hit
TCACGTCGTTCC1605110.9001449835105301No Hit
ACCAAGGGCACT1586280.8895851277750956No Hit
CAGTACGATCTT1517500.8510133339629242No Hit
CGGGCATAAGTC1237140.693787569014097No Hit
GAACTGTATCTC1156490.6485590844117182No Hit
AATGAGCCCACG1149590.6446895674401569No Hit
ACACGGTGTCTA183410.10285624750058645No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTAGC1202.5567715E-86.0014311
CGTTACC1303.6270649E-96.0014311
GGTATCC1202.5567715E-86.0014311
GAGCAGC1405.16593E-106.0014311
TGGGACT650.00125839786.0014311
GGTTAGC600.00338761286.0014311
AGGTCGA1507.2759576E-116.0014311
CGGCAGC953.416144E-66.00143051
TCGGACT1950.06.00143051
ACGGACC852.4361552E-56.00143051
CGGTCGT3450.06.00143051
CGTTAGC1001.281227E-66.001431
TGGCAGC1101.8070568E-76.001431
GGATTAC550.0091383596.001431
GGACAGC2550.06.001431
GCACTGT1351.36788E-96.001431
GCACCCG3650.05.99971443
GAGCGCT4100.05.99971444
AGCGCAC2050.05.99971445
GCCACGG2050.05.99971444
Produced by FastQC (version 0.11.2)