FastQCFastQC Report
Thu 4 Jan 2018
000000000-B4NWY_l01n02_e4_wholepop_270.352000000b0c62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4NWY_l01n02_e4_wholepop_270.352000000b0c62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences778708
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCT3750.018.2607575
AACAGCT3750.018.2607575
CCCTGAG250.007581250318.2607563
TCTGCGG250.007581250318.2607563
ATTTATG250.007581250318.2607563
GTGTTTG452.4352557E-618.2607563
GCCCCTG300.001007662618.2607563
ATTTAGG300.001007662618.2607563
ACAACGC950.018.2607564
GTCATGC950.018.2607564
CTTCGCG300.001007662618.2607563
ATCCTAG250.007581250318.2607563
TGTTTAG452.4352557E-618.2607563
AGACTTG250.007581250318.2607563
CTTCGAG250.007581250318.2607563
TATCAAG300.001007662618.2607563
TGTCACG300.001007662618.2607563
GAAATAG250.007581250318.2607563
GGAATGC658.276402E-1018.2607564
GAGGAGC250.007581250318.2607564
Produced by FastQC (version 0.11.2)