FastQCFastQC Report
Thu 4 Jan 2018
000000000-B4NWY_l01n02_e4_facs4_outgrowth.352000000b0c49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4NWY_l01n02_e4_facs4_outgrowth.352000000b0c49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2793761
Sequences flagged as poor quality0
Sequence length151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCACG351.3510312E-418.2553222
CGGCGTT351.3510312E-418.2553222
CATATTA351.3510312E-418.2553221
ATGATTT351.3510312E-418.2553222
CGTATGC351.3510312E-418.2553223
CGAAATC554.492722E-818.2553223
GCGAATA401.814548E-518.2553222
GCGGTAA351.3510312E-418.2553222
GGTCCAT351.3510312E-418.2553221
GATTTTT351.3510312E-418.2553221
AATGGGG401.814548E-518.2553221
CTGACAG351.3510312E-418.2553222
CTGGTTA401.814548E-518.2553222
GTTACCG351.3510312E-418.2553221
AGGTTTA401.814548E-518.2553222
ATATAAG351.3510312E-418.2553222
GGCGGGC351.3510312E-418.2553223
ACATGAT351.3510312E-418.2553221
CTACAAG401.814548E-518.2553222
CGAGAAC351.3510312E-418.2553223
Produced by FastQC (version 0.11.2)