FastQCFastQC Report
Thu 4 Jan 2018
000000000-B4NWY_l01n02_e4_facs3_outgrowth.352000000b0c3c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4NWY_l01n02_e4_facs3_outgrowth.352000000b0c3c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74168
Sequences flagged as poor quality0
Sequence length151
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTGT309.932194E-418.2683755
CGTTTGT351.3214415E-418.2683755
CTTCATG250.007500324418.2683754
ACTGTTT5500.018.2683757
ATCCTGT309.932194E-418.2683755
GGATGTT401.7652175E-518.2683756
TCCTTTG309.932194E-418.2683754
CAATGTT1450.018.2683756
GTTTGTT1200.018.2683756
TTGTTGT351.3214415E-418.2683755
AACGTGT309.932194E-418.2683755
CATTGTT1300.018.2683756
AAACTGT351.3214415E-418.2683755
TTCATGT701.05501385E-1018.2683755
GCCTTTG250.007500324418.2683754
CCAATGT351.3214415E-418.2683755
CACACTG250.007500324418.2683754
ACGCTGT309.932194E-418.2683755
TAGTGTT657.8216544E-1018.2683756
GTCATGT250.007500324418.2683755
Produced by FastQC (version 0.11.2)