FastQCFastQC Report
Thu 4 Jan 2018
000000000-B4NWY_l01n02_e4_facs2_outgrowth.352000000b0c2f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4NWY_l01n02_e4_facs2_outgrowth.352000000b0c2f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences779920
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG250.00756076618.2693313
TCTGCCG250.00756076618.2693313
TTTAGTG300.001004493718.2693313
TATCATG300.001004493718.2693313
GTTGAGC300.001004493718.2693314
TGTTTGC900.018.2693314
TTTAGGA250.00756076618.2693312
TTTAGCG250.00756076618.2693313
AAGTATG250.00756076618.2693313
TATCAAT300.001004493718.2693312
AAGTAGC606.035407E-918.2693314
GAGGAGC250.00756076618.2693314
CTATGCG300.001004493718.2693313
TTAGGAG300.001004493718.2693313
GAGTGGC452.4243673E-618.2693314
GGGGAGC250.00756076618.2693314
CCTTATG503.2765456E-718.2693313
AACAACG250.00756076618.2693313
AACCGGG250.00756076618.2693313
AGCAAGC452.4243673E-618.2693314
Produced by FastQC (version 0.11.2)