FastQCFastQC Report
Thu 4 Jan 2018
000000000-B4NWY_l01n02_e4_facs1_outgrowth.352000000b0c12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4NWY_l01n02_e4_facs1_outgrowth.352000000b0c12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2089817
Sequences flagged as poor quality0
Sequence length151
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTGG351.3506124E-418.2556953
ATGGGTG351.3506124E-418.2556953
TGTCAGG300.001010314318.2556953
CTAGAAG701.1459633E-1018.2556953
AGCCGTG300.001010314318.2556953
CTATGAC300.001010314318.2556951
AGACTAG351.3506124E-418.2556953
AGGTAGC606.1081664E-918.2556954
GGCAGGC1400.018.2556954
AAATCCT351.3506124E-418.2556951
AGATCAG351.3506124E-418.2556953
GCGACAG554.4899934E-818.2556953
AGTTTAA300.001010314318.2556952
ATAGGTG351.3506124E-418.2556953
GATAAAC351.3506124E-418.2556951
GGTAAGG351.3506124E-418.2556953
GGGCTGC1250.018.2556954
CGAGTGG351.3506124E-418.2556953
CCGATTG606.1081664E-918.2556953
GAAGACG300.001010314318.2556953
Produced by FastQC (version 0.11.2)