FastQCFastQC Report
Thu 4 Jan 2018
000000000-B4NWY_l01n02_d4_wholepop_270.352000000b0c7f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4NWY_l01n02_d4_wholepop_270.352000000b0c7f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2107617
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGGT351.3550383E-418.2479131
ACTATGA300.001013214718.2479131
CTGTCGC554.5120032E-818.2479133
GAAGCTT300.001013214718.2479131
CAAACAA7450.018.2479135
AGTTTTA300.001013214718.2479132
TTAATTG300.001013214718.2479131
AAGAGTC351.3550383E-418.2479133
CAACGTA300.001013214718.2479132
GGTCGAT300.001013214718.2479132
CGCGGCT300.001013214718.2479132
CTATCGT351.3550383E-418.2479132
CTATCAG351.3550383E-418.2479131
GCGAAAC300.001013214718.2479133
TCCTGAT300.001013214718.2479131
CAGTTCG300.001013214718.2479131
GGACTCT300.001013214718.2479131
GAGTACC300.001013214718.2479133
GACCGCT300.001013214718.2479131
GCAGTAC401.8205525E-518.2479133
Produced by FastQC (version 0.11.2)