FastQCFastQC Report
Thu 4 Jan 2018
000000000-B4NWY_l01n02_c4_facs4_outgrowth.352000000b0c05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4NWY_l01n02_c4_facs4_outgrowth.352000000b0c05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences597778
Sequences flagged as poor quality0
Sequence length151
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTGT351.3536369E-418.246764
CTGCTTG452.451221E-618.246763
GTCATTG250.00761283118.246763
CTTCGTG452.451221E-618.246763
TTTAGTA3650.018.246765
TTCTATC250.00761283118.246762
ATTTACG351.3536369E-418.246763
CTTAACG351.3536369E-418.246763
ATTTAAG351.3536369E-418.246763
CATGGTA2000.018.246765
GTGTTAG250.00761283118.246763
CGTGGTA1100.018.246765
GAGTGTA1350.018.246765
CGGGTGT351.3536369E-418.246764
ACTATTG351.3536369E-418.246763
AACGTAT10150.018.246766
AAACTCG250.00761283118.246763
CGTGGGT401.8179948E-518.246764
CATGGAG250.00761283118.246763
AGCCGTA2100.018.246765
Produced by FastQC (version 0.11.2)