FastQCFastQC Report
Thu 4 Jan 2018
000000000-B4NWY_l01n02_c4_facs3_outgrowth.352000000b0bf7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4NWY_l01n02_c4_facs3_outgrowth.352000000b0bf7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1267991
Sequences flagged as poor quality0
Sequence length151
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGCGT92950.018.5743947
GTGTTTG250.006874237718.5743923
TATTTGT250.006874237718.5743922
TATTTCG308.991809E-418.5743923
GTGTTCC351.1823831E-418.5743921
AACAATG452.0715488E-618.5743923
GTGCACG250.006874237718.5743923
TAGAGTG250.006874237718.5743923
AACCGTG502.7567694E-718.5743923
AGCAATG308.991809E-418.5743923
TGATATG604.9240043E-918.5743923
CGTGGGG351.1823831E-418.5743924
AGCGTCC900.018.5743929
CTATGCG351.1823831E-418.5743923
GAGTGGC3250.018.5743925
CAGCGGC4550.018.5743925
GATACTG351.1823831E-418.5743923
AACCGGC7600.018.5743925
CTCACTT250.006874237718.5743921
GTCGCTG250.006874237718.5743923
Produced by FastQC (version 0.11.2)