FastQCFastQC Report
Thu 4 Jan 2018
000000000-B4NWY_l01n02_c4_facs2_outgrowth.352000000b0bea.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4NWY_l01n02_c4_facs2_outgrowth.352000000b0bea.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3017396
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGAGG8300.018.2524595
CGAGAGG4250.018.2524595
GCAGAGG5650.018.2524595
CTTGTTG300.001011660118.2524571
ATTTAAC658.3491614E-1018.2524572
GAGGATA351.3527014E-418.2524573
AACCGTG300.001011660118.2524572
GAGGAGG2500.018.2524575
CTATGCT300.001011660118.2524572
TAGAGGG29000.018.2524576
AACCGGC300.001011660118.2524572
TAATAAT250.00760608818.2524571
AGGTAAG1500.018.2524574
GGGGAAA250.00760608818.2524573
CAGCGAC250.00760608818.2524572
ACTATCG250.00760608818.2524571
ACAGTTT606.1245373E-918.2524572
ACTATAT300.001011660118.2524572
CGGCGCT351.3527014E-418.2524571
GGCAGGG29400.018.2524576
Produced by FastQC (version 0.11.2)