FastQCFastQC Report
Thu 4 Jan 2018
000000000-B4NWY_l01n02_c4_facs1_outgrowth.352000000b0bdd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4NWY_l01n02_c4_facs1_outgrowth.352000000b0bdd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1044979
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACCC250.007560126518.27033
TATTTTT250.007560126518.27032
CTTGTAA300.001004446618.27031
CTGCTAA250.007560126518.27032
TATCACC351.3415492E-418.27033
CTATGGC250.007560126518.27033
CGTGGCT606.037226E-918.27034
TTAGGCC452.4247402E-618.27033
AGCGTAC351.3415492E-418.27033
CAGCGGC300.001004446618.27033
GGGGACT300.001004446618.27034
CGGAAAC401.8000272E-518.27033
ACTATCG250.007560126518.27032
GACAGCC300.001004446618.27033
TGGAGCT351.3415492E-418.27034
GGCAGCT401.8000272E-518.27034
TGCCCTA300.001004446618.27031
ACAGTCC452.4247402E-618.27033
GTACCCC351.3415492E-418.27033
GGAGCTT1500.018.27035
Produced by FastQC (version 0.11.2)