FastQCFastQC Report
Thu 4 Jan 2018
000000000-B4NWY_l01n02_ancestor_barcode_techrep2.352000000b0c8c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4NWY_l01n02_ancestor_barcode_techrep2.352000000b0c8c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences905818
Sequences flagged as poor quality0
Sequence length151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTCA1050.018.2761674
CAAGTCA1050.018.2761674
CAGCCCA1050.018.2761674
AGCACCA503.2651405E-718.2761654
CCCTGAC300.00100214418.2761653
GAAATTC300.00100214418.2761653
GTGCACA503.2651405E-718.2761654
AACGTCA801.8189894E-1218.2761654
CTATGCA401.7946315E-518.2761654
TTAGGAC250.00754539218.2761653
GAGTGCG250.00754539218.2761651
GAGTGCA351.3380052E-418.2761654
CGGAACA751.4551915E-1118.2761654
GGTACTC300.00100214418.2761653
AGCCGCA701.10958354E-1018.2761654
CAAACTC300.00100214418.2761653
CTGTCAA300.00100214418.2761652
GCCTTCT300.00100214418.2761651
CCCCTCG250.00754539218.2761651
TCTTGCG250.00754539218.2761651
Produced by FastQC (version 0.11.2)