FastQCFastQC Report
Thu 4 Jan 2018
000000000-B4NWY_l01n01_e4_facs4_outgrowth.351000000b0c4c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4NWY_l01n01_e4_facs4_outgrowth.351000000b0c4c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2793761
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGCAG151.2658174E-4143.965961
CGGACAG151.2658174E-4143.965962
GTTGAGA151.2658174E-4143.965961
CTGCTCG151.2658174E-4143.965962
CAGGTCG151.2658174E-4143.965962
TGTCAGG306.8394E-10143.965961
CGTGGCG151.2658174E-4143.965962
AGCCGTG151.2658174E-4143.965962
GAGTGCA151.2658174E-4143.965961
TGGAGCG151.2658174E-4143.965961
AGCCGGA151.2658174E-4143.965961
TGGAGAG151.2658174E-4143.965961
TCTGAAG151.2658174E-4143.965961
CCGGGAA151.2658174E-4143.965961
ATGATTG151.2658174E-4143.965962
GATGTAG151.2658174E-4143.965961
GAGCTGT151.2658174E-4143.965961
TTGGAAG151.2658174E-4143.965961
TGCCACG151.2658174E-4143.965962
GAGTCCG151.2658174E-4143.965961
Produced by FastQC (version 0.11.2)