FastQCFastQC Report
Thu 4 Jan 2018
000000000-B4NWY_l01n01_e4_facs3_outgrowth.351000000b0c3f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4NWY_l01n01_e4_facs3_outgrowth.351000000b0c3f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74168
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCCCG450.0143.961585
ACCGGGC18000.0143.961588
AAACCCG351.2732926E-11143.961585
CAGTCCG450.0143.961585
ATCTCCG450.0143.961585
TAAACCG351.2732926E-11143.961585
AGCTCCG351.2732926E-11143.961585
TGCGCCG351.2732926E-11143.961585
TGACCGG900.0143.961586
TTACCCG450.0143.961585
TTTCCCG450.0143.961585
TTATCCG700.0143.961585
CGCTCCG351.2732926E-11143.961585
GAGCCGG450.0143.961586
GTTTCCG351.2732926E-11143.961585
CCCACCG351.2732926E-11143.961585
TCTACCG351.2732926E-11143.961585
GACACCG351.2732926E-11143.961585
TTCCCGG1400.0143.961586
TGCCCGG900.0143.961586
Produced by FastQC (version 0.11.2)