FastQCFastQC Report
Thu 4 Jan 2018
000000000-B4NWY_l01n01_e4_facs2_outgrowth.351000000b0c22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4NWY_l01n01_e4_facs2_outgrowth.351000000b0c22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences779920
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCAC151.2652004E-4143.973662
TCACGGT253.871355E-8143.973663
TTCTATC151.2652004E-4143.973662
GCCCCAC253.871355E-8143.973662
TTTAGCT306.8394E-10143.973663
ATCCTAA151.2652004E-4143.973662
TTCTAGC151.2652004E-4143.973661
GTGCACC306.8394E-10143.973661
TATCAAC151.2652004E-4143.973662
TGTCAGC151.2652004E-4143.973661
AGCGTGG1900.0143.973665
GGGTGTG253.871355E-8143.973664
CGGCGTG253.871355E-8143.973664
GGCAGTG151.2652004E-4143.973664
CAGCGGA253.871355E-8143.973666
TCTGATG500.0143.973664
GATACTC151.2652004E-4143.973662
TTGGCGC151.2652004E-4143.973661
CCGGGTA151.2652004E-4143.973661
ATCTCCA151.2652004E-4143.973662
Produced by FastQC (version 0.11.2)