FastQCFastQC Report
Thu 4 Jan 2018
000000000-B4NWY_l01n01_e4_facs1_outgrowth.351000000b0c15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4NWY_l01n01_e4_facs1_outgrowth.351000000b0c15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2089817
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGCAC100.007245252143.96282
TCGCCTC202.208677E-6143.96281
GTGCATG100.007245252143.96282
GCCCCGA100.007245252143.96281
CTGCTAG202.208677E-6143.96282
GAGGATG100.007245252143.96282
TATCAGG100.007245252143.96281
GAGTGTC100.007245252143.96282
GGGGATC100.007245252143.96281
CAGCGTC100.007245252143.96281
CGGGTAC202.208677E-6143.96282
AGCGTCG202.208677E-6143.96281
CGTGGCG100.007245252143.96281
CGTGGCC100.007245252143.96281
GAGGACT100.007245252143.96281
GGCAGTA100.007245252143.96281
GAGGACC100.007245252143.96282
CGTGGAG100.007245252143.96282
AGGTAGC100.007245252143.96281
TCTGATG100.007245252143.96282
Produced by FastQC (version 0.11.2)