FastQCFastQC Report
Thu 4 Jan 2018
000000000-B4NWY_l01n01_c4_facs4_outgrowth.351000000b0c08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4NWY_l01n01_c4_facs4_outgrowth.351000000b0c08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences597778
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAAAAT100.0072434037143.966552
AGGTCAC100.0072434037143.966553
CCACAAG100.0072434037143.966551
AGCACCG100.0072434037143.966551
TCACGCG100.0072434037143.966552
TCCGGAC100.0072434037143.966553
ACAACTC202.2070872E-6143.966553
ACAACTA202.2070872E-6143.966551
GTCATTT100.0072434037143.966552
TCGCCGC100.0072434037143.966553
ATTTAGT100.0072434037143.966552
TATTTGG202.2070872E-6143.966551
TATTTCG100.0072434037143.966551
ACAACGC400.0143.966553
GTTGACA351.2732926E-11143.966554
CTGCTCT100.0072434037143.966551
TTGATCG100.0072434037143.966552
CTGCTCG100.0072434037143.966552
GTCATGC202.2070872E-6143.966553
TGTCATT100.0072434037143.966552
Produced by FastQC (version 0.11.2)