FastQCFastQC Report
Thu 4 Jan 2018
000000000-B4NWY_l01n01_c4_facs3_outgrowth.351000000b0bfa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4NWY_l01n01_c4_facs3_outgrowth.351000000b0bfa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1267991
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCTTG1055000.0143.836361
GAATTCG1077900.0143.799337
AGCTTGA1059450.0143.748632
AATTCGA1080350.0143.713128
ATTCGAA1080200.0143.713079
TGAATTC1078550.0143.61926
TTGAATT1076750.0143.598565
CTTGAAT1080550.0142.707154
ATGCGAT180300.0142.142979
CATGCGA60550.0140.175088
TATGCGA49900.0138.35328
TCATGCG16450.0137.852867
TGTTGGG218550.0136.99643145
GCATGCG11050.0136.813237
GATGCGA30700.0135.772348
TTATGCG15200.0133.560077
AATGCGA53650.0133.110738
ACATGCG16550.0131.800117
TGATGCG8550.0130.507877
CTATGCG15950.0130.439247
Produced by FastQC (version 0.11.2)